El-Refaey, R., El-Seidy, E., Soliman, I., Fouad, S., El-Nahrawy, S. (2014). HISTOLOGICAL STUDIES ON SOME EGYPTIAN CLOVER GENOTYPES ATTACHED WITH DODDER (Cuscuta spp.). Journal of Plant Production, 5(7), 1115-1128. doi: 10.21608/jpp.2014.56498
R. A. El-Refaey; E. H. El-Seidy; I. E. Soliman; Samira A. Fouad; Shereen M. El-Nahrawy. "HISTOLOGICAL STUDIES ON SOME EGYPTIAN CLOVER GENOTYPES ATTACHED WITH DODDER (Cuscuta spp.)". Journal of Plant Production, 5, 7, 2014, 1115-1128. doi: 10.21608/jpp.2014.56498
El-Refaey, R., El-Seidy, E., Soliman, I., Fouad, S., El-Nahrawy, S. (2014). 'HISTOLOGICAL STUDIES ON SOME EGYPTIAN CLOVER GENOTYPES ATTACHED WITH DODDER (Cuscuta spp.)', Journal of Plant Production, 5(7), pp. 1115-1128. doi: 10.21608/jpp.2014.56498
El-Refaey, R., El-Seidy, E., Soliman, I., Fouad, S., El-Nahrawy, S. HISTOLOGICAL STUDIES ON SOME EGYPTIAN CLOVER GENOTYPES ATTACHED WITH DODDER (Cuscuta spp.). Journal of Plant Production, 2014; 5(7): 1115-1128. doi: 10.21608/jpp.2014.56498
HISTOLOGICAL STUDIES ON SOME EGYPTIAN CLOVER GENOTYPES ATTACHED WITH DODDER (Cuscuta spp.)
1Agronomy Dept., Faculty of Agriculture, Tanta Univ., Egypt.
2Weed Res. Central Lab., Agric. Res.Cent., Giza, Egypt.
3Botany Dept., Faculty of Agriculture, Kafr El-Sheikh Univ., Egypt.
4Forage Crops Res. Dept., Field Crops Res. Inst., ARC, Egypt.
Abstract
Three field experiments were carried out at Sakha Agricultural Research Station Farm, Kafer El-Sheikh Governorate, ARC, Egypt, during three successive winter seasons of 2008/09, 2009/10 and 2010/11, to investigate the performance of some egyption clover genotype for its resistance/tolerance to dodder infestation. Anatomical structures as well as biochemical analyses for berseem plants werecarried out toidentify variation inthe correlated traits with tolerance to dodder infestation. The results indicated that genotypes no. 35 and 65 showed that the moderate genotypes behaved different, the parasite is scarcely able to pierce the epidermis but lignifications of host pericycle and starch sheath showed incompatible interaction between clover genotypes and C. planiflora. The sensitive genotypes no. 29 and 94 were in contrast compatible with the parasite, the infection developed normally with the intrusive cells reaching the center cylinder and the host vascular tissues.
The results indicated that the amount of phenolic acids is relatively high; 1111.65 and 965.24 mg/100 mg of foliage dry weight for genotypes; Helali and no. 51, respectively. These two genotypes could retain some tolerance to the infestation with C. planiflora. Whereas, the phenolic acids in the genotypes no. 35 and 74 which are considered to some extent retain moderate tolerance to infestation with dodder were present in lesser concentration (784.6 and 735.38 mg/100 mg). While, Genotypes no. 94 and 29 which could be considered susceptible to infestation with dodder, phenolic acids were found in trace amounts (386.64 and 423.02 mg/100 mg), respectively. HPLC analysis revealed the presence of protocatechuic acid, caffeic acid, P-hydroxyl benzoic acid, coumaric acid, ferulic acid, vanllic acid and syringic acid and their concentrations were different from berseem genotype to another.
The obtained results may be encouraging to use the tolerant genotypes, which had the least reduction percentages due to dodder infestation, for developing compost or synthetic cultivar(s) which retain tolerance to dodder infestation. This cultivar could be used by itself as a way of control of dodder in farmers' fields or integrated with the other factors of dodder control.
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