Okasha,, K., Ragab, M., EI-Domyati, F., Helal, R., Mohamed, N. (2003). PRODUCTION OF NEW STRAWBERRY STRAINS VIA ANTHER CULTURE: 3- THE CYTOlOGYCAL STUDY AND DNA FINGERPRINTINg. Journal of Plant Production, 28(6), 4449-4461. doi: 10.21608/jpp.2003.244642
Kh. A. Okasha,; M. E. Ragab; F. EI-Domyati; R. Helal; N. A. Mohamed. "PRODUCTION OF NEW STRAWBERRY STRAINS VIA ANTHER CULTURE: 3- THE CYTOlOGYCAL STUDY AND DNA FINGERPRINTINg". Journal of Plant Production, 28, 6, 2003, 4449-4461. doi: 10.21608/jpp.2003.244642
Okasha,, K., Ragab, M., EI-Domyati, F., Helal, R., Mohamed, N. (2003). 'PRODUCTION OF NEW STRAWBERRY STRAINS VIA ANTHER CULTURE: 3- THE CYTOlOGYCAL STUDY AND DNA FINGERPRINTINg', Journal of Plant Production, 28(6), pp. 4449-4461. doi: 10.21608/jpp.2003.244642
Okasha,, K., Ragab, M., EI-Domyati, F., Helal, R., Mohamed, N. PRODUCTION OF NEW STRAWBERRY STRAINS VIA ANTHER CULTURE: 3- THE CYTOlOGYCAL STUDY AND DNA FINGERPRINTINg. Journal of Plant Production, 2003; 28(6): 4449-4461. doi: 10.21608/jpp.2003.244642
PRODUCTION OF NEW STRAWBERRY STRAINS VIA ANTHER CULTURE: 3- THE CYTOlOGYCAL STUDY AND DNA FINGERPRINTINg
This study was carried out dUring 1998 to 2002 to establish an efficient protocol for generating somaclonal variations in strawberry via anther culture ard selecting the promising ones for the strawberry breeding programs in Egypt. T~n strains from Camarosa. and a strain from each of Capitola and Chandler were subjected to nursery and fruit produc:tlon trials in a randomized complete blocks design. Cytological studies and DNA f-ngerprintings were carried out 'or the selected
strains. I
Ail examined root lips of all selected strains showed that the chromosome number was 56 (octaploid) as in the original cultivars. The pairwise differences among strawberry genotypes were calculated by (SPSS). The analysis was based on the number of mac1c.el'l that were different between any given pair of genotypes. lihe results of analysis showed two groups. The highest value of similarity was observed between the two Camarosa strains C8 and C9; which dustered together and closely grouped with Chandler strain 61. and the three genotypes could also be grouped "Yith Camaro$8 Cultivar. Also, C5 and C6 Camarosa strains Showed a second grqup together. with a high similarity value, and they were clustered with Camarosa strain C10, and the three strains couid be grouped with Camarosa strain C3. Moreo er, Camarosa strain C4 was dosely grouped with the last mentioned four strains.
The study conclude that, consensus fingerprint prOf.ing using the rando Iy amplified polymorphic DNA (RAPO) mac1cers is a useful and reliable method for establishing genetic identities of the strawberry cultivars and advanced selecti ns. regenerated from anther culture.
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