Emara, H., Ibrahim, I., EL-Massry, M., Dahab, A. (2008). BIOTECHNOLOGICAL STUDIES ON Solanum viride PLANT: A- IN VITRO PROPAGATION OF Solanum viride PLANT THROUGH TISSUE CULTURE TECHNIQUE.. Journal of Plant Production, 33(3), 2133-2148. doi: 10.21608/jpp.2008.135766
H. A. Emara; I. A. Ibrahim; M. H. EL-Massry; A. A. Dahab. "BIOTECHNOLOGICAL STUDIES ON Solanum viride PLANT: A- IN VITRO PROPAGATION OF Solanum viride PLANT THROUGH TISSUE CULTURE TECHNIQUE.". Journal of Plant Production, 33, 3, 2008, 2133-2148. doi: 10.21608/jpp.2008.135766
Emara, H., Ibrahim, I., EL-Massry, M., Dahab, A. (2008). 'BIOTECHNOLOGICAL STUDIES ON Solanum viride PLANT: A- IN VITRO PROPAGATION OF Solanum viride PLANT THROUGH TISSUE CULTURE TECHNIQUE.', Journal of Plant Production, 33(3), pp. 2133-2148. doi: 10.21608/jpp.2008.135766
Emara, H., Ibrahim, I., EL-Massry, M., Dahab, A. BIOTECHNOLOGICAL STUDIES ON Solanum viride PLANT: A- IN VITRO PROPAGATION OF Solanum viride PLANT THROUGH TISSUE CULTURE TECHNIQUE.. Journal of Plant Production, 2008; 33(3): 2133-2148. doi: 10.21608/jpp.2008.135766
BIOTECHNOLOGICAL STUDIES ON Solanum viride PLANT: A- IN VITRO PROPAGATION OF Solanum viride PLANT THROUGH TISSUE CULTURE TECHNIQUE.
1Dept. of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Egypt.
2Dept. of Medicinal and Aromatic Plants, Horticulture Research Institute.
Abstract
Leaves of Green nightshade (Solanum viride Solander ex Forst. f.) family: Solanaceae were used as source of explants to start micropropagation. Leaves were successfully sterilized using the treatment of sodium hypochlorite (NaOCl) at 1.0% that showed the highest percentage of survival without any contamination. The highest fresh weight of callus/explant was recorded when the leaf segments were cultured on MS media contained 0.5 mg/l BA and 1.5 mg/l NAA. Interestingly, some treatments recorded direct organogenesis (direct shoots and roots) from the leaf explants. The highest direct shoot formation was recorded with MS media supplemented with 2 mg/l BA alone. However, it was clear that all shoots obtained with the responded treatments were vitrified. Therefore, in a trial to obtain unvitrified shoots as indirect organogenesis through callus formation and differentiation, all indirect obtained shoots were vitrified except some shoots (4.7 % of them) obtained on MS media supplemented with 1.0 mg/l BA alone. These unvitrified shoots were successfully acclimatized in soil mixture of sand and peatmoss (1:2, v: v) that showed the best growth and percentage of survival (95%).
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