Rabie, K., Abd El-Aal, M., Manaf, H. (2010). ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.. Journal of Plant Production, 1(2), 319-332. doi: 10.21608/jpp.2010.86356
Kawthar A. E. Rabie; Mona S. Abd El-Aal; H. H. Manaf. "ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.". Journal of Plant Production, 1, 2, 2010, 319-332. doi: 10.21608/jpp.2010.86356
Rabie, K., Abd El-Aal, M., Manaf, H. (2010). 'ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.', Journal of Plant Production, 1(2), pp. 319-332. doi: 10.21608/jpp.2010.86356
Rabie, K., Abd El-Aal, M., Manaf, H. ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.. Journal of Plant Production, 2010; 1(2): 319-332. doi: 10.21608/jpp.2010.86356
ENHANCED SILYMARIN ACCUMULATION AS INFLUENCE OF MEDIUM COMPOSITION IN CELL SUSPENSION CULTURES OF Silybum marianum (L.) GAERTN.
Agric. Botany Dept., Fac. of Agric., Ain Shams Univ., Shoubra El-Keima, Cairo, Egypt.
Abstract
Silymarin production was examined in cell suspension cultures derived from hypocotyl of Silybum marianum (L.) Gaertn. MS medium supplemented with different concentrations of sucrose, glucose, KH2PO4, KH2PO4 + K2SO4, NH4NO3, CaCl2, L-Phenylalanine, L-Tyrosine and yeast extract was used. Tyrosine 10 mg/l, 0.5 KH2PO4 + K2SO4 con., 0.75 NH4NO3 con., 0.75 KH2PO4 + K2SO4 con., glucose 30 g/l, 0.5 CaCl2.2H2O, tyrosine 20 mg/l and yeast extract 40 mg/l (3 days incubation period) showed the highest production of silymarin in callus cells to reach 79%, 57.5%, 46%, 24.2%, 21.2%, 19.7%, 9.7% and 8.6% over the control respectively. The same trend was obtained by HPLC (qualitative assay).
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