El-Namaky, R. (2012). IDENTIFICATION OF SOME WIDELY COMPATIBLE RESTORER LINES USING TESTCROSS AND SSR MARKERS. Journal of Plant Production, 3(11), 2913-2926. doi: 10.21608/jpp.2012.85355
R. A. El-Namaky. "IDENTIFICATION OF SOME WIDELY COMPATIBLE RESTORER LINES USING TESTCROSS AND SSR MARKERS". Journal of Plant Production, 3, 11, 2012, 2913-2926. doi: 10.21608/jpp.2012.85355
El-Namaky, R. (2012). 'IDENTIFICATION OF SOME WIDELY COMPATIBLE RESTORER LINES USING TESTCROSS AND SSR MARKERS', Journal of Plant Production, 3(11), pp. 2913-2926. doi: 10.21608/jpp.2012.85355
El-Namaky, R. IDENTIFICATION OF SOME WIDELY COMPATIBLE RESTORER LINES USING TESTCROSS AND SSR MARKERS. Journal of Plant Production, 2012; 3(11): 2913-2926. doi: 10.21608/jpp.2012.85355
IDENTIFICATION OF SOME WIDELY COMPATIBLE RESTORER LINES USING TESTCROSS AND SSR MARKERS
Rice Research &Training Center (RRTC), Sakha Kafr El-Sheikh, 33717 - Egypt
Abstract
Hybrids between distantly related high yielding varieties are most promising. Discovery and exploitation of widely compatible varieties provide a possibility for the utilization of heterosis between Indica and Japonica types. Investigation was carried out at the farm of Rice Research &Training Center (RRTC), Sakha Kafr El-Sheikh, Egypt from 2008 at 2010. To evaluate the presence of restorer and wide compatibility genes for 85 lines of F5 developed from the cross between Giza178 and Dular. Six SSR markers were used to detect the presence of restoring ability (Rf) and wide compatibility (WC) genes. A total of 19 lines selected by SSR markers were crossed as male (testers) with two Indica cytoplasmic male sterile lines (IR58025A and IR69625A) and two Japonica CVS; Sakha101 and Giza177 as female parents to produce 76 F1 hybrids seeds according to 4 x19 Line x Tester fashion. Results of SSR markers indicated that Dular variety had two alleles (S-5 and S-8) for wide compatibility (WC) While, Giza178 had two Rf alleles; Rf-3 and Rf-4. However, the selected lines from F5 generations holded at least one Rf allele either Rf3 or Rf4 and one WC allele either S-5 or S-8. Some lines had the four alleles, two alleles (Rf3 and Rf4) for restoring ability, and two alleles (S-5 and S-8) for wide compatibility. All 19 testers showed high pollen and spikelets fertility% (more than 80%) with Japonica testers, Sakha101 and Giza177 while, 13 lines showed pollen and spikelets fertility more than 80%. with Indica CMS lines (IR58025A and IR69625A). These results indicated that WC alleles and restoring ability alleles (Rf) are present in these lines and these lines can be used to produce Indica/ Japonica hybrids. For heterosis of grain yield plant-1, most of crosses exhibited significant heterosis with positive values ranged between 17.31 and 59.34 %.
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