Metwally, E., El-Denary, M., Kasem, M. (2015). EFFECT OF GAMMA RAYS ON CALLUS GROWTH AND PLANTLET REGENERATION IN GARLIC. Journal of Plant Production, 6(9), 1571-1579. doi: 10.21608/jpp.2015.52022
E. I. Metwally; M. E. El-Denary; M. H. Kasem. "EFFECT OF GAMMA RAYS ON CALLUS GROWTH AND PLANTLET REGENERATION IN GARLIC". Journal of Plant Production, 6, 9, 2015, 1571-1579. doi: 10.21608/jpp.2015.52022
Metwally, E., El-Denary, M., Kasem, M. (2015). 'EFFECT OF GAMMA RAYS ON CALLUS GROWTH AND PLANTLET REGENERATION IN GARLIC', Journal of Plant Production, 6(9), pp. 1571-1579. doi: 10.21608/jpp.2015.52022
Metwally, E., El-Denary, M., Kasem, M. EFFECT OF GAMMA RAYS ON CALLUS GROWTH AND PLANTLET REGENERATION IN GARLIC. Journal of Plant Production, 2015; 6(9): 1571-1579. doi: 10.21608/jpp.2015.52022
EFFECT OF GAMMA RAYS ON CALLUS GROWTH AND PLANTLET REGENERATION IN GARLIC
1Horticulture Department, Faculty of Agriculture, Kafrelsheikh University.
2Research Institute, Agriculture Research Center, Giza, Egypt.
Abstract
This study was carried out in the Tissue Culture Laboratory, Horticulture Department, Faculty of Agriculture, KafrEl-SheikhUniversity in 2014 for increasing genetic variability plants. Fresh callus (21 days old) derived from root tips segments from Balady cv. was exposed to nine doses of gamma rays (0, 50, 100, 150, 200, 250, 500, 1000 and 2000 rad). After 21 days from radiation treatments, low doses of gamma rays stimulated callus growth, while higher doses decreased them. Low doses of gamma rays (50 rad) produced the highest callus fresh weight, while, 2000 rad produced the lowest value. After 60 days from radiation treatments, the effect of radiation was continuously noticed. Callus fresh weight was increased with increasing the radiation dose from 50 to 100 rad, and then decreased with increasing gamma irradiation doses. Therefore the lowest value of callus fresh weight was recorded at 2000 rad. Gamma rays at 100 rad produced the lowest differentiated plantlets per callus (normal, abnormal and total). Callus produced 100% normal plantlets at the low doses of radiation, i.e., from 50 to 250 rad, however some of abnormal plantlets were produced at the higher doses of gamma rays. Percentage of abnormal plantlets increased by increasing gamma ray doses until 500 rad which produced the highest record (18.1%).
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