In vitro PROPAGATION OF SOME CITRUS ROOTSTOCKS. 1- EFFECT OF EXPLANT SOURCE AND GROWING MEDIA GROWTH REGULATORS CONTENT.

El-Boray, M. S. S.; Mustafa, M. F. M.; Shalan, A. M. N.; Abed, A. A.

doi:10.21608/jpp.2015.49791

In vitro PROPAGATION OF SOME CITRUS ROOTSTOCKS. 1- EFFECT OF EXPLANT SOURCE AND GROWING MEDIA GROWTH REGULATORS CONTENT.

Document Type : Original Article

Authors

Hort. Dep. Fac. Of Agric. Mansura Uni.

10.21608/jpp.2015.49791

Abstract

This investigation was carried out during 2013 to 2015 seasons in tissue culture laboratory of Horticulture Department, Agriculture Faculty, Mansoura University, to make comparssion between shoot tips and epicotyls explants in vitro propagation of five Citrus rootstocks namely, Sour orange (Citrus aurantium, L.), Volkamer lemon (Citrus volkmeriana, Ten.). Cleopatra mandarin (Citrus reshni, Blanco), Rangpur lime and Troyer citrange . Our results showed that, epicotyl of Rangur rootstock, Sour orange and Volkamariana which cultured on MS medium containing BAP+Kin (BAP at 1 mg/L, Kin at 1.5 mg/L) gave distinct significant increase of length of shoots/plantlet from epicotyl explants comparing with another tested treatment in this study. Also, explants of epicotyl that took from Volkamariana, Sour orange and Rangpur on MS medium supplemented with IBA+NAA(IBA at 2 mg/L, NAA at 2mg/L) in vitro had significant increase of length of roots/explant comparing with other two citrus rootstocks in this respect.

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