Effect of LITHOVIT and Nano-Titanium Additives in Rooting Stag Amelioration of Plant Date Palm (type Khalas) by Tissue Culture Field

Khater, M. S.; Mohamed, Mervat H.; Mansour, L. L.; Mohamed, A. M.

doi:10.21608/jpp.2024.315542.1375

Effect of LITHOVIT and Nano-Titanium Additives in Rooting Stag Amelioration of Plant Date Palm (type Khalas) by Tissue Culture Field

Document Type : Original Article

Authors

¹ National Institute of Laser Enhanced Science (NILES), Cairo University, Giza, Egypt.

² Tissue culture and plant transformation DPT, Agricultural genetic engineering research institute (AGERI). Agric. Res. Center (ARC), Giza ,Egypt

³ Dept. of Date Palm Pests and Diseases, Central Lab. for Date Palm, Agric. Res. Center (ARC), Giza ,Egypt.

⁴ The Central Laboratory for Date Palm Researches and Development, Res. Center (ARC), Giza ,Egypt.

10.21608/jpp.2024.315542.1375

Abstract

The current work aims to integrate LITHOVIT with nano-titanium to enhance the date palm tissue culture micro-propagation technique.Titanium (Ti) significantly impacts plants biologically. At low concentrations, Ti is beneficial, but at large ones, it is harmful.To feed explants with a medium at different stages of tissue culture propagation of the Khalas Date palm variety (Phoenix dactylifera) (L.), buds explants were inoculated on MS medium + 0.1 mg/l of (IAA) + varying levels of LITHOVIT (organic fertiliser) and Nano-Titanium Dioxide (nano fertiliser).Morphological statistics reveal that the addition of LITHOVIT (0.75 g/l) has the highest significant number with Nano-Titanium Dioxide (2 ppm) in the (leaf length, leaf number/explant, root number/explant, and root length). On the other hand, chemical statistical analyses show that LITHOVIT (0.75 g/l) and Nano-Titanium Dioxide (2 ppm) are significant for total sugar and Indols. Still, LITHOVIT (1 g/l) without Nano-Titanium Dioxide has the highest significant number for total phenols.

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