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Journal of Plant Production
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El-Shereif, A., Abou Elyazid, D. (2024). Effectiveness of Colchicine in Inducing Polyploidy in 'Balady' Mandarin. Journal of Plant Production, 15(9), 519-523. doi: 10.21608/jpp.2024.314029.1368
A. R. El-Shereif; Doaa M. Abou Elyazid. "Effectiveness of Colchicine in Inducing Polyploidy in 'Balady' Mandarin". Journal of Plant Production, 15, 9, 2024, 519-523. doi: 10.21608/jpp.2024.314029.1368
El-Shereif, A., Abou Elyazid, D. (2024). 'Effectiveness of Colchicine in Inducing Polyploidy in 'Balady' Mandarin', Journal of Plant Production, 15(9), pp. 519-523. doi: 10.21608/jpp.2024.314029.1368
El-Shereif, A., Abou Elyazid, D. Effectiveness of Colchicine in Inducing Polyploidy in 'Balady' Mandarin. Journal of Plant Production, 2024; 15(9): 519-523. doi: 10.21608/jpp.2024.314029.1368

Effectiveness of Colchicine in Inducing Polyploidy in 'Balady' Mandarin

Article 7, Volume 15, Issue 9, September 2024, Page 519-523  XML PDF (459.9 K)
Document Type: Original Article
DOI: 10.21608/jpp.2024.314029.1368
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Authors
A. R. El-Shereif email orcid ; Doaa M. Abou Elyazidorcid
Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, 33516 kafr El-Sheikh, Egypt.
Abstract
Lateral buds of potted mandarin plants (Citrus reticulata Balnco cv 'Balady') were treated with four concentrations of colchicine (0.0, 0.1, 0.5 and 1.0%) for five consecutive days in order to investigate colchicine effectiveness in producing tetraploid plants of important mandarin cultivar in Egypt. These tetraploid plants can later be used as parents for producing seedless triploid plants through hybridization with diploid ones. Colchicine treatments decreased bud sprouting percentage and reduced shoot length. Furthermore, increasing colchicine concentration reduced the number of stomata per unit area compared to the control; however, stomatal length and width increased proportionally. Based on chromosome counting, the highest tetraploid percentage (42.2%) was obtained at 1.0% colchicine followed by the treatment of 0.5% (35.6%), whereas 0.1% concentration achieved the lowest tetraploid percentage (11.3%). The tetraploid shoots exhibited a broader leaf area and bigger floral buds expressed in length and width compared to the diploid mother plants. The results indicate that 1.0% colchicine is the most effective concentration for producing tetraploid plants.
Keywords
Chromosome doubling; Citrus reticulata; tetraploid; stomata number
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