El-Weshy, E., El-Naggar, N., Haroun, S., Sherief, A. (2018). Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production. Journal of Plant Production, 9(11), 919-922. doi: 10.21608/jpp.2018.36604
Eman M. El-Weshy; Noura E. El-Naggar; S. A. Haroun; A. A. Sherief. "Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production". Journal of Plant Production, 9, 11, 2018, 919-922. doi: 10.21608/jpp.2018.36604
El-Weshy, E., El-Naggar, N., Haroun, S., Sherief, A. (2018). 'Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production', Journal of Plant Production, 9(11), pp. 919-922. doi: 10.21608/jpp.2018.36604
El-Weshy, E., El-Naggar, N., Haroun, S., Sherief, A. Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production. Journal of Plant Production, 2018; 9(11): 919-922. doi: 10.21608/jpp.2018.36604
Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production
1Department of Botany, Faculty of Science, Mansoura University, Egypt
2Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria 21934, Egypt
Abstract
The main objective of this study was screening of some fungal strains for their ability to produce uricase and selecting the most potent isolatesfor medically important uricase production. Twenty-five fungal strains were screened for their potentialities for uricase production by uricase plate assay method on uric acid medium. After incubation, the formation of clear zone around the fungal colony indicated its potentiality for uricase production. Among the tested strains of fungi using the plate assay method, Emericella quadrilineata, Aspergillus welwitschiae, Aspergillus carenus and Aspergillus flavus were the most active fungal strains which were able to produce uricase. These fungal strains were tested for uricase production under submerged fermentation conditions. The highest uricase activity in liquid medium containing uric acid was obtained from Emericella quadrilineata (23.38 U/mL), Aspergillus welwitschiae (19.87 U/mL), Aspergillus carenus (16.37 U/mL) and Aspergillus flavus (10.91 U/mL).The optimum conditions for maximum Emericella quadrilineata and Aspergillus welwitschiae uricase production were at temperature of 30oC, uric acid concentration of 3 g /L and pH 6.5.
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