Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production

El-Weshy, Eman M.; El-Naggar, Noura E.; Haroun, S. A.; Sherief, A. A.

doi:10.21608/jpp.2018.36604

Screening of some Fungal Isolates for their Potentialities for Uricase Production and Optimization of the Factors Affecting the Production

Document Type : Original Article

Authors

¹ Department of Botany, Faculty of Science, Mansoura University, Egypt

² Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications, Alexandria 21934, Egypt

10.21608/jpp.2018.36604

Abstract

The main objective of this study was screening of some fungal strains for their ability to produce uricase and selecting the most potent isolatesfor medically important uricase production. Twenty-five fungal strains were screened for their potentialities for uricase production by uricase plate assay method on uric acid medium. After incubation, the formation of clear zone around the fungal colony indicated its potentiality for uricase production. Among the tested strains of fungi using the plate assay method, Emericella quadrilineata, Aspergillus welwitschiae, Aspergillus carenus and Aspergillus flavus were the most active fungal strains which were able to produce uricase. These fungal strains were tested for uricase production under submerged fermentation conditions. The highest uricase activity in liquid medium containing uric acid was obtained from Emericella quadrilineata (23.38 U/mL), Aspergillus welwitschiae (19.87 U/mL), Aspergillus carenus (16.37 U/mL) and Aspergillus flavus (10.91 U/mL).The optimum conditions for maximum Emericella quadrilineata and Aspergillus welwitschiae uricase production were at temperature of 30^oC, uric acid concentration of 3 g /L and pH 6.5.

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