Sakr, S., Taha, H. (2000). COMPARATIVE STUDIES ON Hypericum perforatum L. IN VIVO AND IN VITRO. Journal of Plant Production, 25(10), 6397-6408. doi: 10.21608/jpp.2000.259797
Salwa S. Sakr; H. S. Taha. "COMPARATIVE STUDIES ON Hypericum perforatum L. IN VIVO AND IN VITRO". Journal of Plant Production, 25, 10, 2000, 6397-6408. doi: 10.21608/jpp.2000.259797
Sakr, S., Taha, H. (2000). 'COMPARATIVE STUDIES ON Hypericum perforatum L. IN VIVO AND IN VITRO', Journal of Plant Production, 25(10), pp. 6397-6408. doi: 10.21608/jpp.2000.259797
Sakr, S., Taha, H. COMPARATIVE STUDIES ON Hypericum perforatum L. IN VIVO AND IN VITRO. Journal of Plant Production, 2000; 25(10): 6397-6408. doi: 10.21608/jpp.2000.259797
COMPARATIVE STUDIES ON Hypericum perforatum L. IN VIVO AND IN VITRO
1Department of Ornamental Horticulture, Faculty of Agriculture, Cairo University, Giza, Egypt.
2Department of Plant Cell and Tissue Culture, National Research Centre , Dokki, Giza, Egypt.
Abstract
The effect of normal and continuous day light (White lamps 3000 Lux) as well as, cold treated or untreated seeds on propagation of Hypericum perforatum L. In vivo and In vitro were studied. It was found that: In vivo, the cold treated seeds with 4°C for 30 days before cultivation under continuous light showed the best results, i.e. higher germination percentage; plant height (cm); greater number of branches/plant; fresh and dry weight (gm/plant) and dry matter content (%) as compared with untreated seeds which cultured either under normal or continuous day light. In vitro studies, the obtained plantlets from cultivation of cold treated seeds on MS-agar medium supplemented with 0.05 mg/l NAA + 0.5 mg/l BA, and incubated at 26 ± 1°C for three months then, transferred into greenhouse under continuous day light, showed the best results on obvious determinations than untreated seeds or treated seeds and cultured under normal day light. Propagation of Hypericum perforatum L In Vitro showed better results than In Vivo.
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