Sakr, S., Dessouky, M. (2000). PRODUCTION OF TROPANE ALKALOIDS IN Atropa belladonna L. USING CELL CULTURE TECHNIQUE .. Journal of Plant Production, 25(10), 6383-6396. doi: 10.21608/jpp.2000.259794
Salwa S. Sakr; M. M. Dessouky. "PRODUCTION OF TROPANE ALKALOIDS IN Atropa belladonna L. USING CELL CULTURE TECHNIQUE .". Journal of Plant Production, 25, 10, 2000, 6383-6396. doi: 10.21608/jpp.2000.259794
Sakr, S., Dessouky, M. (2000). 'PRODUCTION OF TROPANE ALKALOIDS IN Atropa belladonna L. USING CELL CULTURE TECHNIQUE .', Journal of Plant Production, 25(10), pp. 6383-6396. doi: 10.21608/jpp.2000.259794
Sakr, S., Dessouky, M. PRODUCTION OF TROPANE ALKALOIDS IN Atropa belladonna L. USING CELL CULTURE TECHNIQUE .. Journal of Plant Production, 2000; 25(10): 6383-6396. doi: 10.21608/jpp.2000.259794
PRODUCTION OF TROPANE ALKALOIDS IN Atropa belladonna L. USING CELL CULTURE TECHNIQUE .
1Department of Horticulture, Faculty of Agriculture, Cairo University, Giza, Egypt.
2National Organization for Drug Control and Research (NODCAR ), Giza, Egypt.
Abstract
A promising protocol for enhancement of total alkaloids production from cell cultures of A. belladonna L. was established. The effect of MS-medium supplemented with different concentrations from 2,4-D and Kin or NAA and BA on both of growth parameters as well as total alkaloids production were investigated. The effect of various concentrations of phenylalanin and ornithine as a precursors of alkaloids accumulation were studied. The optimum value of cell growth parameters and alkaloids production were obtained from leaf, stem and root cell cultures, respectively. The most effective supplementations to liquid MS-medium (Murashige and Skoog,1962) were 1 mg/l from each of NAA and BA. Phenylalanine at 20 mg/l gave the highest value of cell growth and alkaloids accumulation in the different types of cell cultures. Total alkaloids of different types cultures were identified using HPLC.
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