GREEN GLOBULER BODIES INDUCATION OF Platycerium bifurcatum THROUGH TISSUE CULTURE

Plantlets of Platycerium bifurcatum were successfully regenerated  from leaf section via callus formation, GGB (green globular bodies) production as intermediate stage and GGB segments were proliferation into shoots.
         Fraible callus induction in leaf section was achieved on full strength MS medium supplemented with 1 mg/L^-1 2,4- D+1mg/l kinetin within two subculture (every 4 weeks). The maximum production of GGB was obtained by the addition of 3 mg/l BA to MS medium. Segments of GGB were cultured on MS medium containing relatively low concentration of NAA (0.0 to 0.9 mg/l) and regenerate to larger shoot numbers than GGB segments which were cultured on medium containing relatively high concentrations of auxins NAA, IBA, IAA (5,10 and 15 mg/l).
         The greatly shoot numbers production from GGB segments when 0.9 mg/L^1- NAA was added to the medium. Higher growth value and shoot numbers were observed on GGB segments by using mediuum supplemented with 0.9 mg/l NAA, solidified by 3.0 mg/l agar and adjusted pH to 5.2. Also, the effect of agar concentration and pH value were examined. Shoots were planted in plastic pots containing peatmoss sand mixture (3:1v/v) during acclimatization in greenhouse.