THE RELATIONSHIP BETWEEN GROWTH REGULATORS AND THE OXIDATIVE STRESS INDUCTION IN SOYBEAN NODULES GROWN UNDER SALINE CONDITIONS

Document Type : Original Article

Authors

1 Botany Department National Research Centre, Dokki, Cairo, Egypt

2 Agricultural Microbiology Department National Research Centre, Dokki, Cairo, Egypt

Abstract

Effect of pre-soaking of  soybean seeds in GA3 or IAA treatments on number, dry weight, nitrogen, protein, leghaemoglobin, MDA contents and SOD activity in nodules of soybean Glycine max (L.) Merr. cv. Crawford was studied under different salinity levels (control, 6 and 12 dS/m) in a greenhouse experiment.
The studied parameters were severely inhibited due to irrigation with saline water and the impact was more obvious at  the vegetative stage. Application of growth regulators, reduced the determined effect of salinity on nodulation, nitrogen, protein and leghaemoglobin contents and decreased the extent of membrane damage which was monitored by measuring the MDA content. On the contrary, the SOD activity was not affected by IAA or GA3 treatments
Abbreviations:  
Lb     = Leghaemoglobin       SOD = Super-oxide dismutase      AOS = Active oxygen species
MDA = Malondialdehyde      TBA = 2-Thiobarbutyric acid     ROS = Reactive oxygen species

Keywords