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Abd El-Halim,, M., Abd El-Salam, M., Ibrahim, S., Abd-El-Aal, S., Attallah, A. (2001). GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION. Journal of Plant Production, 26(9), 5427-5436. doi: 10.21608/jpp.2001.257774
M. M. Abd El-Halim,; M. S. Abd El-Salam; S. A. Ibrahim; S. Kh. Abd-El-Aal; A. G. Attallah. "GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION". Journal of Plant Production, 26, 9, 2001, 5427-5436. doi: 10.21608/jpp.2001.257774
Abd El-Halim,, M., Abd El-Salam, M., Ibrahim, S., Abd-El-Aal, S., Attallah, A. (2001). 'GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION', Journal of Plant Production, 26(9), pp. 5427-5436. doi: 10.21608/jpp.2001.257774
Abd El-Halim,, M., Abd El-Salam, M., Ibrahim, S., Abd-El-Aal, S., Attallah, A. GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION. Journal of Plant Production, 2001; 26(9): 5427-5436. doi: 10.21608/jpp.2001.257774

GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION

Article 8, Volume 26, Issue 9, September 2001, Page 5427-5436  XML PDF (676.77 K)
Document Type: Original Article
DOI: 10.21608/jpp.2001.257774
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Authors
M. M. Abd El-Halim,1; M. S. Abd El-Salam1; S. A. Ibrahim2; S. Kh. Abd-El-Aal1; A. G. Attallah1
1Microbial Genetics Dept., National Research Center, Cairo, Egypt
2Genetics Dept., Faculty of Agriculture Ain Shams Univ., Cairo, Egypt
Abstract
Two distinguished Bradyrhizobium japonicum Egyptian strains, i.e. SE8 and DO4, each was fused with the fast growing Rhizobium fredii USDA201 strain. Nine fusants were obtained in the first attempt (USDA 201 and SE8) and  four fusants were obtained in the second attempt ( USDA201 and DO4). All fusants were characterized on the bases of nodulation efficiency, salt and drought tolerance, heavy metals resistance and antibiotic resistance. Electrophoretic plasmid profiles of these fusants and their parents were studied and the relatedness between them was determined according to their phylogeneric tree. Some fusants retained their nodulation efficiency and showed higher efficiencies in salt, drought and /or heavy metals resistance than their parents.
Keywords
Bradyrhizobium japonicum; Rhizobium fredii; Genetic improvement; Intergeneric; Protoplast fusion; Phylogenetic analysis
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