ETHANOL 	PRODUCTION 	FROM CELLULOSE VIA INTERGENERIC 	PROTOPLAST FUSION 	BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS

Talkhan,, Fatma N.; EI-Bondkly, A. M.; Ismail, A. A.

doi:10.21608/jpp.2002.257032

ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS

Document Type : Original Article

Authors

Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt.

10.21608/jpp.2002.257032

Abstract

The present study aimed to investigate the possibility of inducing new
genetic recombinants having the ability to ferment cellulose into ethanol upon fusion
of T. reese; and S. cerevisiae protoplasts. Twelve stable fusants were induced and
lested for ethanol as well as cellulase enzymes productivity. Out of these fusants 8
isolates produced ethanol with amounts ranged from 45% (F/4 and F/5) to 90% (Ff7)
of the parent S. cerevisiee, whereas the remaing 4 did not produce ethanol. Meantime
Ihese none ethanol producing fusants showed cellulase activities of the three
enzymes (FPase, CMCase and ~-9Iucosidase).
On th other hand, 3 fusants (F/1, F/6 and Fn) out of the eight ethanol
producer showed cellulase activities of these three enzymes, whereas the other five
fusants (F/2, F/4, F/5, F/9 and F/10) showed cellulase activities of the two enzymes
FPar.e and CMCase only. None of these 12 fusants overyieled the parental strain T.
reese; (3126) in cellulases production.

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