Talkhan,, F., EI-Bondkly, A., Ismail, A. (2002). ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS. Journal of Plant Production, 27(11), 7335-7345. doi: 10.21608/jpp.2002.257032
Fatma N. Talkhan,; A. M. EI-Bondkly; A. A. Ismail. "ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS". Journal of Plant Production, 27, 11, 2002, 7335-7345. doi: 10.21608/jpp.2002.257032
Talkhan,, F., EI-Bondkly, A., Ismail, A. (2002). 'ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS', Journal of Plant Production, 27(11), pp. 7335-7345. doi: 10.21608/jpp.2002.257032
Talkhan,, F., EI-Bondkly, A., Ismail, A. ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS. Journal of Plant Production, 2002; 27(11): 7335-7345. doi: 10.21608/jpp.2002.257032
ETHANOL PRODUCTION FROM CELLULOSE VIA INTERGENERIC PROTOPLAST FUSION BETWEEN Trichoderma reese; AND Saccharomyces cerevisiae FUSANTS
Genetics and Cytology Department, National Research Center, Dokki, Cairo, Egypt.
Abstract
The present study aimed to investigate the possibility of inducing new genetic recombinants having the ability to ferment cellulose into ethanol upon fusion of T. reese; and S. cerevisiae protoplasts. Twelve stable fusants were induced and lested for ethanol as well as cellulase enzymes productivity. Out of these fusants 8 isolates produced ethanol with amounts ranged from 45% (F/4 and F/5) to 90% (Ff7) of the parent S. cerevisiee, whereas the remaing 4 did not produce ethanol. Meantime Ihese none ethanol producing fusants showed cellulase activities of the three enzymes (FPase, CMCase and ~-9Iucosidase).
On th other hand, 3 fusants (F/1, F/6 and Fn) out of the eight ethanol producer showed cellulase activities of these three enzymes, whereas the other five fusants (F/2, F/4, F/5, F/9 and F/10) showed cellulase activities of the two enzymes FPar.e and CMCase only. None of these 12 fusants overyieled the parental strain T. reese; (3126) in cellulases production.
View on SCiNiTO
Top