Hussein,, E., Aly,, A., Mohamed, M., Zayed, S., Abou-Zeid, T. (2002). LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW. Journal of Plant Production, 27(6), 3781-3792. doi: 10.21608/jpp.2002.254905
E. M. Hussein,; A. A. Aly,; Maggie E. Mohamed; S. M. E. Zayed; T. A. Abou-Zeid. "LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW". Journal of Plant Production, 27, 6, 2002, 3781-3792. doi: 10.21608/jpp.2002.254905
Hussein,, E., Aly,, A., Mohamed, M., Zayed, S., Abou-Zeid, T. (2002). 'LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW', Journal of Plant Production, 27(6), pp. 3781-3792. doi: 10.21608/jpp.2002.254905
Hussein,, E., Aly,, A., Mohamed, M., Zayed, S., Abou-Zeid, T. LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW. Journal of Plant Production, 2002; 27(6): 3781-3792. doi: 10.21608/jpp.2002.254905
LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW
1Plant Pathology Research Institute, Agricultural Res. Center, Giza, Egypt.
2Field Crops Research Institute, Agricultural Research Center, EI- Gemmeiza, Egypt.
Abstract
Proteins of ten flax genotypes having different levels of susceptibility to powdery mildew (PM) were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SOS-PAGE). Gels were stained with Coomassie Brillian Blue R-250 (CBB) or silver nitrate (SN). Cluster analsysis of the protein banding patterns by the unweighted pair-group method based on arithmetic means (UPGMA) placed the genotypes in several groups with overall similarity levels ranged from 66.56 for SN to 95.65% for CBB, which indicates that SN was more sensitive than CBB to detect the differences among the genotypes in protein banding patterns. Grouping the cultivars based on their protein banding patterns stained with SN was not related to their susceptibility to PM. The results of this study indicate that cultivar specific bands may be useful as biochemical markers for cultivar identification or for seed purity tests.