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Journal of Plant Production
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Volume Volume 26 (2001)
Volume Volume 25 (2000)
Hussein,, E., Aly,, A., Mohamed, M., Zayed, S., Abou-Zeid, T. (2002). LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW. Journal of Plant Production, 27(6), 3781-3792. doi: 10.21608/jpp.2002.254905
E. M. Hussein,; A. A. Aly,; Maggie E. Mohamed; S. M. E. Zayed; T. A. Abou-Zeid. "LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW". Journal of Plant Production, 27, 6, 2002, 3781-3792. doi: 10.21608/jpp.2002.254905
Hussein,, E., Aly,, A., Mohamed, M., Zayed, S., Abou-Zeid, T. (2002). 'LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW', Journal of Plant Production, 27(6), pp. 3781-3792. doi: 10.21608/jpp.2002.254905
Hussein,, E., Aly,, A., Mohamed, M., Zayed, S., Abou-Zeid, T. LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW. Journal of Plant Production, 2002; 27(6): 3781-3792. doi: 10.21608/jpp.2002.254905

LACK OF RELATIONSHIP BETWEEN PROTEIN ELECTROPHORETIC PATTERNS OF FLAX GENOTYP-ES AND THEIR SUSCEPTIBILITY TO POWDERY MILDEW

Article 11, Volume 27, Issue 6, June 2002, Page 3781-3792  XML PDF (3.05 MB)
Document Type: Original Article
DOI: 10.21608/jpp.2002.254905
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Authors
E. M. Hussein,1; A. A. Aly,1; Maggie E. Mohamed1; S. M. E. Zayed1; T. A. Abou-Zeid2
1Plant Pathology Research Institute, Agricultural Res. Center, Giza, Egypt.
2Field Crops Research Institute, Agricultural Research Center, EI- Gemmeiza, Egypt.
Abstract
Proteins of ten flax genotypes having different levels of susceptibility to powdery
mildew (PM) were compared by sodium dodecyl sulfate-polyacrylamide gel
electrophoresis (SOS-PAGE). Gels were stained with Coomassie Brillian Blue R-250
(CBB) or silver nitrate (SN). Cluster analsysis of the protein banding patterns by the
unweighted pair-group method based on arithmetic means (UPGMA) placed the
genotypes in several groups with overall similarity levels ranged from 66.56 for SN to
95.65% for CBB, which indicates that SN was more sensitive than CBB to detect the
differences among the genotypes in protein banding patterns. Grouping the cultivars
based on their protein banding patterns stained with SN was not related to their
susceptibility to PM. The results of this study indicate that cultivar specific bands may
be useful as biochemical markers for cultivar identification or for seed purity tests.
Keywords
Flax genotypes; powdery mildew; SOS-PAGE
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