PRODUCTION OF SALT-TOLERANT SOUR ORANGE PLANTS (Citrus aurantium L.) USING TISSUE CULTURE TECHNIQUE: III- EMBRYOGENESIS INDUCTION FROM SALT-TOLERANT CALLUS

Document Type : Original Article

Authors

Department of Pomology, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

Abstract

This study was conducted through 1996-1999 years in the Department of Pomology, Faculty of Agriculture, Alexandria University. This work involved induction of somatic embryogenesis, from the salt-tolerant callus and subsequently, production of salt- tolerant plants of sour orange.
The main results can be summarized in the following points:
1-  The MT (1969) medium containing 1500mgl-1 malt extract exhibited significantly embryogenesis percentage  (60.00 %) higher than that obtained from the other tested media. The MT (1969) medium was generally more effective than MS (1962) medium in inducing somatic embryogenesis from salt-tolerant callus of sour orange.
2-The percentage of fully grown embryoids was significantly the highest (33.33%) on the MT (1969) medium containing 1mgl-1 GA3.