PLANT REGENERATION FROM LEAF BLADE, PEDICLE AND SHOOT TIP OF CULANTRO (Eryngium foetidum L.), A MEDICINAL PLANT

Document Type : Original Article

Author

Genetic Engineering and Biotechnology Research Institute Sadat City, P.O, Box 79122857, Minufiya University, Egypt

Abstract

Culantro is an aromatic plant. which is used as condiment and medicinal
plant. A procedure for shoe; regeneration (rom leaf blade, pedicle and shoot tip
explants, is described. Leaf blade, pedicle and shool tip explants were cultured on
Murashige and Skoog medium (MS) alone or supplernentert with 0,5,1,2,3,4,5,6
mgll benzyladenine (SA) with 0.5 mgll naphlhaleneacetic acid (NAA). Shoot
regeneration from the proximal third section of leaf blade was higher than shoot
regeneration from the middle and distal sections. Shoot regeneration, shoot length
and roots number were innuenced by SA and NAA concentrations. Shoot
regeneration from quartered shoot tip was higher than shoot regeneration from
decapitated or intact shoot tip. Produced shoots were transferred into root- induction
medium and rooted shoots were successfully established in soil.
Abbreviations: SA, benzyladenine; MS, Murashige & Skoog's (1962) medium; NAA •
naphthaleneacetic acid.

Keywords