INDUCED MUTATIONAL VARIABILITY BY GAMMA- RIl.DIATION OR ETHYLMETHANE SULPHONATE AND THEIR COMBINATIONS IN PEA Pisum sativum L.

Document Type : Original Article

Authors

1 Vegetable and Floriculture Dept., Fac. Agric., Mansoura Univ.

2 Horticulture Department, Fac. Agric., Kafr EI-Sheikh, Tanta Univ.

Abstract

Seeds of the pea cultivar Master B were irradiated with different doses of
gamma-rays (0, 2.5, 5, 10, 15, 20, 25 and 30 KR), another portions of the seeds were
treated with different levels of EMS (0.0, 0.025, 0.05, 0.1 %). Also, there were
combined treatments using the two mutagenic agents. The results of this investigation
showed that the M2-plants exhibited different degrees of reduction in growth in
comparison with those of the untreated control plants. In M2, the mutagenic doses
significantly increased the mean value for plant height, number of pods per plant and
seed yield per plant. However, the results did not show additional variation for number
of branches per plant and number of seeds. per pod. In M2-generation, 107 different
mutant lines were selected.