EI-Sharabasy,, S., EI-Shiaty, O., Zaied, N. (2004). IN VITRO CALLAS INITIATION, FORMATION AND EVALUATION OF SOME DATE PALM (Phoenix dactylifera L.) CUL TIVARS.. Journal of Plant Production, 29(10), 5801-5812. doi: 10.21608/jpp.2004.238864
S. F. EI-Sharabasy,; Olfat H. EI-Shiaty; Nagwa S. Zaied. "IN VITRO CALLAS INITIATION, FORMATION AND EVALUATION OF SOME DATE PALM (Phoenix dactylifera L.) CUL TIVARS.". Journal of Plant Production, 29, 10, 2004, 5801-5812. doi: 10.21608/jpp.2004.238864
EI-Sharabasy,, S., EI-Shiaty, O., Zaied, N. (2004). 'IN VITRO CALLAS INITIATION, FORMATION AND EVALUATION OF SOME DATE PALM (Phoenix dactylifera L.) CUL TIVARS.', Journal of Plant Production, 29(10), pp. 5801-5812. doi: 10.21608/jpp.2004.238864
EI-Sharabasy,, S., EI-Shiaty, O., Zaied, N. IN VITRO CALLAS INITIATION, FORMATION AND EVALUATION OF SOME DATE PALM (Phoenix dactylifera L.) CUL TIVARS.. Journal of Plant Production, 2004; 29(10): 5801-5812. doi: 10.21608/jpp.2004.238864
IN VITRO CALLAS INITIATION, FORMATION AND EVALUATION OF SOME DATE PALM (Phoenix dactylifera L.) CUL TIVARS.
1The Central Lab. of Date Palm Research and Develop., Agric. Res. Centre
2Ornamental Dept., Faculty of Agric. Cairo University.
3Hort. Dept., National Research Center, Dokki, Giza, Egypt
Abstract
Uniform Shoot-tip explants of some female date palm cultivars (Sewy, Zaghloul, Samany, Sakouty and Malakaby) were cultured on modified Murashige and Skoog medium. Different concentrations of auxins i.e 2,4-Dichlorophenoxy acetic acid (2,4-0); Naphthalene acetic acid (NAA) and a-Naphthoxy acetic acid (NOA) were tested.
Data indicated that culturing of shoot-tips as explants on modified Murashige and Skoog medium is mainly recommended. Also, callus initiation of different date palm cvs were maximized by using (100 mg/L 2,4-0). However, the treatments of (10 mg/L 2,4-0 + 10 mg/L NOA + 10 mg/L NAA ) induced the best growth parameters (number of explant, percentage of callus formation and average value of callus formation). Meanwhile, Sewy cultivar had less swelling and callus formation compared with the other cultivars.
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