IN VITRO CALLAS INITIATION, FORMATION AND EVALUATION OF SOME DATE PALM (Phoenix dactylifera L.) CUL TIVARS.

Document Type : Original Article

Authors

1 The Central Lab. of Date Palm Research and Develop., Agric. Res. Centre

2 Ornamental Dept., Faculty of Agric. Cairo University.

3 Hort. Dept., National Research Center, Dokki, Giza, Egypt

Abstract

Uniform Shoot-tip explants of some female date palm cultivars (Sewy,
Zaghloul, Samany, Sakouty and Malakaby) were cultured on modified Murashige and
Skoog medium. Different concentrations of auxins i.e 2,4-Dichlorophenoxy acetic acid
(2,4-0); Naphthalene acetic acid (NAA) and a-Naphthoxy acetic acid (NOA) were
tested.
Data indicated that culturing of shoot-tips as explants on modified Murashige
and Skoog medium is mainly recommended. Also, callus initiation of different date
palm cvs were maximized by using (100 mg/L 2,4-0). However, the treatments of (10
mg/L 2,4-0 + 10 mg/L NOA + 10 mg/L NAA ) induced the best growth parameters
(number of explant, percentage of callus formation and average value of callus
formation). Meanwhile, Sewy cultivar had less swelling and callus formation compared
with the other cultivars.