INDIRECT CLONAL MICROPROPAGATION OF TWO YEMENI LOCAL GRAPE CUL TIVARS FROM SHOOT APICAL MERISTEMS IN VITRO

Elward,, H. O.; Hussein, E. A.; Almaidama, E. M.

doi:10.21608/jpp.2004.238861

INDIRECT CLONAL MICROPROPAGATION OF TWO YEMENI LOCAL GRAPE CUL TIVARS FROM SHOOT APICAL MERISTEMS IN VITRO

Document Type : Original Article

Authors

¹ Horticulture and Forests Department, Faculty of Agriculture, Sana'a University, Sana'a, Republic of Yemen.

² Biology Department, Faculty of Science, Sana'a University, Sana'a, Republic of Yemen.

10.21608/jpp.2004.238861

Abstract

In the present study. trials were done in order to micropropagate two ocal
and popular Yemeni grape Cultivars (Vitis vinifera L. cv. Asemi and cv. Razekl) by
obtaining multiple adventitious shoot bud proliferation in vitro from shoot apical
meristems using MS basal salts culture media containing different benzyl adenine and
2,4 0 concentrations. The experimental part of the study was carried out during
2002/2003 in the laboratories of the faculty of agriculture, Sana'a University. Results
of the present study have revealed that multiple adventitious shoot bud formation was
possible from the two selected cultivars under the effect of 1 or 2 mg/1. benzyl
adenine plus 1 or 2 mg/1. of 2,40 within 4 to 6 weeks of incubation in the dark at room
temperature. It was then possible to obtain regenerants from the adventitious shoot
buds in culture tubes containing hormone free MS media. After acclimatization, the
obtained regenerants were successfully transferred to soil. Results of analysis have
shown that the obtained regenerants were identical to their corresponding controls
morphologically but differed in their leaf chlorophyll contents.