Ghallab,, A., Nafie, A., Abdel- Aziz, R. (2004). SOME PHYSIOLOGICAL STUDIES ON INCREASING WATER STRESS TOLERANT OF MICROPROPAGATEO SUGAR- CANE PLANTS BY USING LASER IRRADIATION.. Journal of Plant Production, 29(5), 2431-2458. doi: 10.21608/jpp.2004.238621
A. M. Ghallab,; A. E. Nafie; R. M. Abdel- Aziz. "SOME PHYSIOLOGICAL STUDIES ON INCREASING WATER STRESS TOLERANT OF MICROPROPAGATEO SUGAR- CANE PLANTS BY USING LASER IRRADIATION.". Journal of Plant Production, 29, 5, 2004, 2431-2458. doi: 10.21608/jpp.2004.238621
Ghallab,, A., Nafie, A., Abdel- Aziz, R. (2004). 'SOME PHYSIOLOGICAL STUDIES ON INCREASING WATER STRESS TOLERANT OF MICROPROPAGATEO SUGAR- CANE PLANTS BY USING LASER IRRADIATION.', Journal of Plant Production, 29(5), pp. 2431-2458. doi: 10.21608/jpp.2004.238621
Ghallab,, A., Nafie, A., Abdel- Aziz, R. SOME PHYSIOLOGICAL STUDIES ON INCREASING WATER STRESS TOLERANT OF MICROPROPAGATEO SUGAR- CANE PLANTS BY USING LASER IRRADIATION.. Journal of Plant Production, 2004; 29(5): 2431-2458. doi: 10.21608/jpp.2004.238621
SOME PHYSIOLOGICAL STUDIES ON INCREASING WATER STRESS TOLERANT OF MICROPROPAGATEO SUGAR- CANE PLANTS BY USING LASER IRRADIATION.
2Sugar Crops Research Institute, Agrlc. Research Center, Giza, Egypt
Abstract
The aim of tne present work was to study the effectiveness of Argon-laser irradiation ( 0.0.40.0.80.0, 120.0 and 160.0 minutes) in improving drought tolerance of the micropropagated sugarcane plants grown under differenllevels of peG (0.0 4.0. 8.0, 12.0 and 16.0 % ). For this reason. experiments were conducted during 2001 and 2002 seasons. The obtained results clearly confirmed the absolute superiority of lower dose (40 min.) of laser irradiation treatment. which significantly increases in the growth parameters of sugarcane grown in vitro ( survival percentage. shoellet length. No. of leaves I shootlet. No. of roots I planUet and fresh as well as dry weight of shootlet ) and ex vitro ( culm length, culm diameter. No. of leaves I plant and shool dry weight ) • yield components ( cane and sugar yield I plant) and juice quality characters ( percentage of sucrose. purity. sugar recovery and total soluble solids) over the untreated control treatment. Also. the sugarcane plantlets derived from shoot tip treated with the lower dose ( 40 min. ) of laser rays were tolerant up to 8 % PEG level and were able to continue their growth under glasshouse conditions till maturity and cane production. The data also revealed that tolerance which was more pronounced as a result of the lower rate of laser rays and was associated with high accumUlation of much more quantities inorganic osrnonca.l.e. N. P • K. Mg and Ca as well as lowest quantities of Na. in addition to considerable accumulation of organic protective osmolytes ( sucrose. proline. amino acids and total soluble phenols). photosynthetic pigments (carotenoids and chlorophylls ) and endogenous hormones ( IAA. GA3 and ABA ) in the sugsrcane g~own in vitro and ex vitro. Such accumulation increased as the PEG level was Increased. Such behavior seems to induce more ability for sugarcane plants to continue their growth till maturity and production of cane even under 8 % PEG level. The obtained data suggested the possibility of successful application of the Argon-laser rays to improve drought tolerance of economic crops such as sugarcane.
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