EI Masry,, H., Abd Rabou, F. (2004). A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.). Journal of Plant Production, 29(1), 371-389. doi: 10.21608/jpp.2004.238478
H. EI Masry,; Farida A. Abd Rabou. "A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)". Journal of Plant Production, 29, 1, 2004, 371-389. doi: 10.21608/jpp.2004.238478
EI Masry,, H., Abd Rabou, F. (2004). 'A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)', Journal of Plant Production, 29(1), pp. 371-389. doi: 10.21608/jpp.2004.238478
EI Masry,, H., Abd Rabou, F. A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.). Journal of Plant Production, 2004; 29(1): 371-389. doi: 10.21608/jpp.2004.238478
A STUDY ON IN VITRO PROPAGATION OF PINEAPPLE (Ananas comosus L. MERR.)
1Tropical And Sub Tropical Fruit Research Department
2Horticulture Research Institute Agriculture Research Center, Giza, Egypt.
Abstract
In several trails for surface sterilization by immersing the explants in Noacl (1 .5.10.15 %) + (1.0 %) Hg CI2 for 10.20.30 minutes. the least contaminated explants were obtained by immersing the explants in NaOcl (15%) + (1.0%) HgCI2 for 30 minutes.During the establishment stage after 3
weeks the explants were cultured on liquid and solid MS media. full and half strength supplemented with SAP at 0 . 1 ,2 • 3 mg/L and ISA at 0 . 0.5 , 1.0 mglL for other 6 weeks. The best multiplication media was full strength liquid MS media supplemented with 1 mglL BAP + 1 mg/L IBA .In rooting stage. shootlet were cultured on media containing 0.5 x strength MS salts. 3 mg/L activated charcoal and combinations of ISA at (0 . 0.2 • 0.5 , 1.0 . 2.0 mglL) and NAA at (0 • 0.2 , 0.5 , 1.0 mglL) • the rooted shootlets were obtained by culturing on MS media supplemented with 1 mg/L NAA + 0.5 mg/L ISA . At hardening stage of 3 months duration by using mixture of peatmoos : sand • the mixture of 3 peatmoss : 1 sand gave the highest percentage of survivals and leaf number.
The acclimatization lasted for 6 months then fertilization experiments on 12m onths old explants were carried out using a mixture of ammonium sulphate (20%) . super phosphate (15% ) and potassium sulphate (48%) in ten treatments as follows: 2:1:2 . 5:1:5 . 6:1:6 , 2:1.5:2 . 5:1.5:5,6:1.5:6. 2:2:2.5:2:5.7:2:7 and 7:1:7 these mixtures were applied to each pots in four doses (0.5 . 1.0 • 1.5 • 2.0 gm) every week during the six months. the highest values of survival percentage. plant length. stem length • stem thickness. number of leaves and leaves area were obtained at ratio of 5:1.5:5 and 7:2:7 using a dose of 1 gm I plant every week.
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