Aboshama, H. (2005). IN VITRO DIRECT PLANT REGENERATION OF Calendula officinalis L.. Journal of Plant Production, 30(12), 7955-7966. doi: 10.21608/jpp.2005.237940
H. M. S. Aboshama. "IN VITRO DIRECT PLANT REGENERATION OF Calendula officinalis L.". Journal of Plant Production, 30, 12, 2005, 7955-7966. doi: 10.21608/jpp.2005.237940
Aboshama, H. (2005). 'IN VITRO DIRECT PLANT REGENERATION OF Calendula officinalis L.', Journal of Plant Production, 30(12), pp. 7955-7966. doi: 10.21608/jpp.2005.237940
Aboshama, H. IN VITRO DIRECT PLANT REGENERATION OF Calendula officinalis L.. Journal of Plant Production, 2005; 30(12): 7955-7966. doi: 10.21608/jpp.2005.237940
IN VITRO DIRECT PLANT REGENERATION OF Calendula officinalis L.
Minufiya University -- Genetic Engineering and Biotechnology Research Institute. Plant Biotechnology Department, Egypt.
Abstract
Regenerating of whole plants of Calendula otticicnslis, L. was achieved through direct organogenesis using leaf and cotyledonary nodes as explants. Leaves were taken from 3·week-old in vitro plantlets and cultured on MS basal medium supplemented with 200 mg/L glutamine and 500 mgl L casein hydrolisate. The medium contained SA with different auxins (2,4-0 and NAA) Prolific direct adventitious shoot regeneration occurred on most of the tested media which that contained either 2.4-0 or NAA. The best response in terms of frequency of shoot regeneration (86.64%), number of shoots per explant (7.60) and bud forming capacity (6.58) were obtained with 1.0 mg/L 2,4-0 alone. Cotyledonary node explants were cultured on MS medium,supplemented with various concentrations of Kin and NAA. The highest frequency of regenerated shoots was achieved on MS medium supplemented with 2.0 mg/L Kin and 1.0mg/L NAA (9.20). Regenerated shoots were excised and rooted, the highest number of root/shoot was obtained with half strength MS salt medium supplemented with 1.0 mg/L ISA In vitro rooted plantlets were finally transferred to mixture of peatmoss and vermiculite al equal volume w·th survival rate95% after 21 days.
Abbreviations: MS- Murashige and Skoog; 2,4-D - diChlorophenoxyacetic acid; BA-Benzyladenine ; NAA- a-naphthalene acetic acid; IBA- indole-3- butyric acid; Kin-Kinetin, BFC; Sud Forming Capacity
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