INTENSIVE PRODUCTION OF DIHAPLOID PLANTS FROM Cucurbita pepo L. THROUGH ANTHER CULTURE TECHNIQUE.

Document Type : Original Article

Authors

1 • Department of Vegetable Crops, Faculty of Agric., Cairo University, Egypt.

2 Horticulture Department, Faculty of Agriculture, Tanta University, Kafr EI-Sheikh, Egypt

Abstract

Production of haploid plants using techniques such as anthers culture has
long been considered important fOf gene~ic studies and plant breeders. Since haploid
plants carry only one allele of each gene, recessive characteristics are apparent.
These haploid plants can be used to produce homozygous dihaploid plants useful for
plant breeding. This studv is concerned with the production of dihaploids plants of
Cucurbita p < /em>epo L. Anthers at mid to late uninucleate microspore stage without
filament were excised from sterilized buds of three genotypes of summer squash: i. e
Gabbla, Eskandarani and Rosina F,. Anthers were cultured on two different induction
media. It was found that both media and cultivars affected callus induction and
plantlets regeneration. The cullivar Gabbla gave the maximum callus weight (0.450 g)
on MS medium supplemented with 5.0 mg II 2.4-0 and 90 gIl sucrose. On the other
hand, the cultivar Eskandarani gave the maximum number of plantlets per dish
(21.3)and per callus (4.3). Plantlets were acclimatized in the controlled environment.
In each genotype, the root tips of plantlets were examined for ploidy level. Results
reveal that there was 50% haploid plants in Eskandarani and Gabbla cultivars while
Rosina F 1 had 40% haploid plants.
Journal of Plant Production
Volume 30, Issue 12
December 2005
Pages 7885-7893

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