lDENTlF1CATION OF DtFFERENT1AL D1SPLAYEO MOLECULES INVOLVED IN EARLINESS AND GROWTH ENHANCEMENT IN TOMATO PLANTS

EI-Halfawi,, Kh. A.; Guirgis, A. A.; EI-Absawy, E. A.; Abd  AI-aziz, Amal A.; Esmaiel, Hany H.

doi:10.21608/jpp.2005.237740

lDENTlF1CATION OF DtFFERENT1AL D1SPLAYEO MOLECULES INVOLVED IN EARLINESS AND GROWTH ENHANCEMENT IN TOMATO PLANTS

Document Type : Original Article

Authors

¹ Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menofiya University

² Plant Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, Menofyia University. Sadat City, Egypt.

10.21608/jpp.2005.237740

Abstract

Three tomato genotypes; late flowering, Lycopersicon Iycopersicum
"Brandywine", early flowering Lycopersicon Iycopersicum "Sweetie" and the wild
type Lycopersicon esculentum "PI 176631", were used to study the effects of both
indole acetic acid (1M) and drought treatments on some morphological and
biochemical characters. The results showed that, 1M treatment increased Doth the
growth rate and protein concentration in the three tomato genotypes and stimulated a
fragment of cDNA isolated from tomato leaf and stem tissues with a molecular weight
of about 740 bp which might be related to auxin regulated gene "LeExp2". PCR
amplified genomic DNA fragment of 1000bp suggested that the tomato varieties
Castle Rock and Super Strain B could be considered as growth nimiety varieties,
while the varieties Peto 86, Floradid and Edcawy could be considered as growth
unanimity varieties.
Drought treatment caused an increment in protein concentration promoted the
flowering in the three tomato genotypes and stimulated the induction of mRNA
isolated from tomato flowers on which a cDNA fragment with of about 750bp was
PCR hunted with specific primers for the flower gene "Tomf 216". Fragment with
molecular weight of about 850bp which may related to Tomf216 gene was PCR
amplified form the three tomato varieties, Castle Rock , peto 86 and Floradid
compared with the same fragment which was isolated form tomato Lycopersicon
esculentum "P1176631 ". These results suggested that these tomato varieties could
considered as an early flowering varieties, while the tomato varieties Super Strain B
and Edcawy could be considered as late flowering varieties.