Document Type : Original Article
Authors
1
Molecular Biology Dept., Genetic Engineering and Biotechnology Research Institute, Minufiya University.
2
Agric. Botany Dept., Faculty of Agriculture, Minufiya University.
3
Bioinformatics Dept., Genetic Engineering and Biotechnology. Research In stitute, Minufiya University.
4
Plant Research Dept., Atomic Energy Authority, Egypt .
Abstract
Twenty-four laba bean mutants were selected and treated with gamma rays
doses of 30 and 60 Gy and ethyl methane sulfonate (EMS)at conc.of 0.15 and 0.30%.
The effect of mutagens was stUdied using 8 number of parameters,such 8S
morphological growth characters, physiological parameters. flOWering, yield attributes
and the genetic characterization ot an protein and DNA molecular markers based on
SOS-PAGE RAPD analysis. The data showed that the induced mutants by either
gamma rays or EMS tended to have higher values in most growth characters and the
maximum increase was achieved by the mutant lines No.7, 14, 24, 25, 18, 19 and 26.
Mutant lines which were produced by (EMS} were more ertec\ive in increasing the
concentrations of photosynthetic pigments, ChI. b, carotenoids and total chlorophylls
then that prcduced by gamma ray. Transpiration rate ( TR) exceeded the control in all
mutants produced by gemma radialion e'Xcept for mutant line No. 18, On the other
hand, mutants No.5, 12. 20, 23, 24 end 25 which were produced by EMS also
exceeded the control. Mutant lines produced by gamma rays were more effective
than that produced by (EMS), As tor shelling percentages, the mutant lines No.7, 8,
9,21, 23 and 18 showed a Significant increase while the mutant lines No. " 4 , 19,
and 26 showed considerable lower velues than the control. The increase in yield and
yield components using physical and chemical mutegens was noticed. II was possible
to associate the specific polymorphic DNA f(agments or about 3000 bp and 200 bp in
size which were generated by the random primers (A1, AS and A3} 101 Gy or EMS
mutants markers might be of great importance in future programs of Vicia tsbe plants
breeding and selection for mutants, witn both high yield and early fiowering .
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