Nassr, M., Selim, A., EI.Absawy, E., Atia, Z., Guirqis, A., Abd El-Aziz, A. (2005). THE ASSOCIATION OF BOTANICAL TRAITS WITH MOLECULAR MARKERS USING INDUCED MUTANTS OF FABA BEAN. Journal of Plant Production, 30(9), 5209-5229. doi: 10.21608/jpp.2005.237477
M, l. Nassr; A. H. Selim; E. A. EI.Absawy; Z. M. Atia; A. A. Guirqis; Amal A. Abd El-Aziz. "THE ASSOCIATION OF BOTANICAL TRAITS WITH MOLECULAR MARKERS USING INDUCED MUTANTS OF FABA BEAN". Journal of Plant Production, 30, 9, 2005, 5209-5229. doi: 10.21608/jpp.2005.237477
Nassr, M., Selim, A., EI.Absawy, E., Atia, Z., Guirqis, A., Abd El-Aziz, A. (2005). 'THE ASSOCIATION OF BOTANICAL TRAITS WITH MOLECULAR MARKERS USING INDUCED MUTANTS OF FABA BEAN', Journal of Plant Production, 30(9), pp. 5209-5229. doi: 10.21608/jpp.2005.237477
Nassr, M., Selim, A., EI.Absawy, E., Atia, Z., Guirqis, A., Abd El-Aziz, A. THE ASSOCIATION OF BOTANICAL TRAITS WITH MOLECULAR MARKERS USING INDUCED MUTANTS OF FABA BEAN. Journal of Plant Production, 2005; 30(9): 5209-5229. doi: 10.21608/jpp.2005.237477
THE ASSOCIATION OF BOTANICAL TRAITS WITH MOLECULAR MARKERS USING INDUCED MUTANTS OF FABA BEAN
1Molecular Biology Dept., Genetic Engineering and Biotechnology Research Institute, Minufiya University.
2Agric. Botany Dept., Faculty of Agriculture, Minufiya University.
3Bioinformatics Dept., Genetic Engineering and Biotechnology. Research In stitute, Minufiya University.
4Plant Research Dept., Atomic Energy Authority, Egypt .
Abstract
Twenty-four laba bean mutants were selected and treated with gamma rays doses of 30 and 60 Gy and ethyl methane sulfonate (EMS)at conc.of 0.15 and 0.30%. The effect of mutagens was stUdied using 8 number of parameters,such 8S morphological growth characters, physiological parameters. flOWering, yield attributes and the genetic characterization ot an protein and DNA molecular markers based on SOS-PAGE RAPD analysis. The data showed that the induced mutants by either gamma rays or EMS tended to have higher values in most growth characters and the maximum increase was achieved by the mutant lines No.7, 14, 24, 25, 18, 19 and 26. Mutant lines which were produced by (EMS} were more ertec\ive in increasing the concentrations of photosynthetic pigments, ChI. b, carotenoids and total chlorophylls then that prcduced by gamma ray. Transpiration rate ( TR) exceeded the control in all mutants produced by gemma radialion e'Xcept for mutant line No. 18, On the other hand, mutants No.5, 12. 20, 23, 24 end 25 which were produced by EMS also exceeded the control. Mutant lines produced by gamma rays were more effective than that produced by (EMS), As tor shelling percentages, the mutant lines No.7, 8, 9,21, 23 and 18 showed a Significant increase while the mutant lines No. " 4 , 19, and 26 showed considerable lower velues than the control. The increase in yield and yield components using physical and chemical mutegens was noticed. II was possible to associate the specific polymorphic DNA f(agments or about 3000 bp and 200 bp in size which were generated by the random primers (A1, AS and A3} 101 Gy or EMS mutants markers might be of great importance in future programs of Vicia tsbe plants breeding and selection for mutants, witn both high yield and early fiowering .
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