Nasr,, M., EI-Halafawy, K., Hamdi, A., EI-Absawy, E., Zayed, E. (2005). TISSUE CULTURE ORGANOGENSISES IN SOYBEAN. Journal of Plant Production, 30(9), 5201-5208. doi: 10.21608/jpp.2005.237474
M. I. Nasr,; K. A. EI-Halafawy; A. Hamdi; E. EI-Absawy; E. M. Zayed. "TISSUE CULTURE ORGANOGENSISES IN SOYBEAN". Journal of Plant Production, 30, 9, 2005, 5201-5208. doi: 10.21608/jpp.2005.237474
Nasr,, M., EI-Halafawy, K., Hamdi, A., EI-Absawy, E., Zayed, E. (2005). 'TISSUE CULTURE ORGANOGENSISES IN SOYBEAN', Journal of Plant Production, 30(9), pp. 5201-5208. doi: 10.21608/jpp.2005.237474
Nasr,, M., EI-Halafawy, K., Hamdi, A., EI-Absawy, E., Zayed, E. TISSUE CULTURE ORGANOGENSISES IN SOYBEAN. Journal of Plant Production, 2005; 30(9): 5201-5208. doi: 10.21608/jpp.2005.237474
1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Egypt.
2Cell Research Department, Field Crops Research Institute, Agricultural Research Center (ARC), Giza, Egypt.
3Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University; Egypt.
Abstract
The aim of this study was to initiate and maintain callus organogenesis cultures of soybean and evaluate the response 01 seven soybean genotypes in callus formation. The genotypes were selected on the basis of their reaction to cotton leaf worm infection. This investigation was conducted in the field at Giza research station in 2003 and 2004seasons. Immature embryos (INith 0.5-10 mm long) were excised from immature seeds and used as ex-plant. The basal Murashige and Skoog (MS) medium with addition of 4 times of micronutrients, Proline (1381 mgll), NAA (0.0372 mg/l) and SAP (2.996 mg/l) gave complete satisfaction for callus production. Formation of shoots and roots were succeeded and rooted plantlets were transplanted to pots in green house. The results indicted that the genotype L86K-73 was the best in response to tissue culture technique in soybean. Callus growth rate was positively and significantly correlaled with each of number 01 shoots/callus and number of roots. Therefore, both characters could be used to predict succeeding of callus groYJth in soybean.
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