TISSUE CULTURE ORGANOGENSISES IN SOYBEAN

Nasr,, M. I.; EI-Halafawy, K. A.; Hamdi, A.; EI-Absawy, E.; Zayed, E. M.

doi:10.21608/jpp.2005.237474

TISSUE CULTURE ORGANOGENSISES IN SOYBEAN

Document Type : Original Article

Authors

¹ Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University, Egypt.

² Cell Research Department, Field Crops Research Institute, Agricultural Research Center (ARC), Giza, Egypt.

³ Bioinformatics Department, Genetic Engineering and Biotechnology Research Institute, Minufiya University; Egypt.

10.21608/jpp.2005.237474

Abstract

The aim of this study was to initiate and maintain callus organogenesis cultures
of soybean and evaluate the response 01 seven soybean genotypes in callus
formation. The genotypes were selected on the basis of their reaction to cotton leaf
worm infection. This investigation was conducted in the field at Giza research station
in 2003 and 2004seasons. Immature embryos (INith 0.5-10 mm long) were excised
from immature seeds and used as ex-plant. The basal Murashige and Skoog (MS)
medium with addition of 4 times of micronutrients, Proline (1381 mgll), NAA (0.0372
mg/l) and SAP (2.996 mg/l) gave complete satisfaction for callus production.
Formation of shoots and roots were succeeded and rooted plantlets were
transplanted to pots in green house. The results indicted that the genotype L86K-73
was the best in response to tissue culture technique in soybean. Callus growth rate
was positively and significantly correlaled with each of number 01 shoots/callus and
number of roots. Therefore, both characters could be used to predict succeeding of
callus groYJth in soybean.