CALLUS iNDUCTION, REGENERATION AND ACTIVE ) t . PRINCIPLE PRODUCTION FROM Urginea maritime (L.) BAKER

EI-Bakry,, A. A.; Soliman, H. M.; Shahen, Heba S.; Ghazi, Salta M.

doi:10.21608/jpp.2006.236431

CALLUS iNDUCTION, REGENERATION AND ACTIVE ) t . PRINCIPLE PRODUCTION FROM Urginea maritime (L.) BAKER

Document Type : Original Article

Authors

¹ Botany Dept Fac. of Sd., Heiwan University

² Pharmacognosy Dept., Fac. of Pharmacy, Helwan University

³ Plant Blotechnològy Department, Genetic Engineering and Biotechnology Inst. Menofya University, Sadat City.

10.21608/jpp.2006.236431

Abstract

The effect of different benzyl adenine (BA) concentrations and bulb explant
zones on regeneration from Lirginea maritime bulbs was studied. Results showed
that Z2 on BA conc. 4.0 mg/i gave the highest mean number of shoots after four
months.
The effect of different 2, 4aD concentrations and explant zones on the
production of both somatic embryos and calli showed that callus production was
highest on 2.0 mg/i 2,40 when using explants of Z2 and Z5.I-lighest frequency of
embryo production on 0.25 mg/i 2,4-D, using Z4. The highest mean number of
embryos was produced when explants of Z1 were treated with 4.0 mgll2, 4-D after 4
months.
Using different naphthalene acetic acid (NAA) concentrations, Callus was
produced with high frequency on 2.0 mg/i when Z2 explants were used and also on
4.0 mg/i when Zland Z4 explants were used.
Mean number of embryos was highest on 1.0 mg/i NAA from ZI explants.
Analysis of variance for the effect of NAA on explant zones showed significant
differences between NAA concentrations, explant zones, and also for their interaction.
Proscillaridin A (PsA) was purified from field collected material using silica gel
columns and the structure was determined and identification carried out by means of
El-MS, ‘HNMR and 13CeNMR.
in vitro culture were initiated on different concentrations of BA and 2, 4-D. Both
caIN and regenerated plants were tested for the presence of cardiac glycosides using
thin layer chromatography (TLC) and quantified for PsA using HPLC. In calli PsA
ranged from 0.10-0.1 8%DW in samples positive for PsA.
Regenerated plants produced through organogenesis were positive for cardiac
glycosides but negative for PsA except bulbs grown for 15 months from Z1 on media
supplemented with 4.0 mg/I BA and 0.1 mg/i NAA which was 0.20% dry weight PsA.