El-Shihy,, O., Ghallab, A., Youssef lbrahim, H., Roder, M. (2006). MOLECULAR DIVERSITY OF FOUR EGYPTIAN WHEAT CULTIVARS REVEALED BY MICROSATELLITES.. Journal of Plant Production, 31(10), 6275-6282. doi: 10.21608/jpp.2006.236333
O. M. El-Shihy,; A. M. Ghallab; H. M. Youssef lbrahim; M. S. Roder. "MOLECULAR DIVERSITY OF FOUR EGYPTIAN WHEAT CULTIVARS REVEALED BY MICROSATELLITES.". Journal of Plant Production, 31, 10, 2006, 6275-6282. doi: 10.21608/jpp.2006.236333
El-Shihy,, O., Ghallab, A., Youssef lbrahim, H., Roder, M. (2006). 'MOLECULAR DIVERSITY OF FOUR EGYPTIAN WHEAT CULTIVARS REVEALED BY MICROSATELLITES.', Journal of Plant Production, 31(10), pp. 6275-6282. doi: 10.21608/jpp.2006.236333
El-Shihy,, O., Ghallab, A., Youssef lbrahim, H., Roder, M. MOLECULAR DIVERSITY OF FOUR EGYPTIAN WHEAT CULTIVARS REVEALED BY MICROSATELLITES.. Journal of Plant Production, 2006; 31(10): 6275-6282. doi: 10.21608/jpp.2006.236333
MOLECULAR DIVERSITY OF FOUR EGYPTIAN WHEAT CULTIVARS REVEALED BY MICROSATELLITES.
1Plant Physiology Dept... Faculty of Agric.. Cairo University. Egypt
2Leibniz Institute of Plant Genetics and Crop Plant Research (IPK). 06466 Gatersleben, Germany
Abstract
The objective of this study was to evaluate the genetic diversity of the most
important Egyptian wheat cultivars grown in 2005 by using microsatellites (SSR) as
molecular markers. The four varieties of wheat (Triticum aestivum L.) tested were
Giza 168. Sids 1, Gemmeza 7 and Gemmeza 9) including Chinese Spring as
standard. The 24 microsatellites used revealed a total of 93 alleles. The fragment size
ranged from 75 bp in GWM3 to 265 bp in GWM931. The average number of alleles
was 3.9 ranging from 2-9 alleles per locus. The average allele number was different
for each cultivar. With 66 alleles the number was highest in Giza 16B, followed by 33
alleles in Bids 1. then Gemmiza 9 with 32 alleles and the lowest allele number was
found in Gimmeza 7 (30 alleles). The polymorphism information content (PIC value)
reflecting the gene diversity of the 24 microsatellite loci ranged from 0.46 to 0.62 with
an average of 0.66. The genetic similarity level ranged from 0.22 for Chinese Spring
with the Egyptian cultivars to 0.58 for Gimmeza 7 and Gimmeza 9. The correlation
coefficient between PIC and number of alleles over 24 microsatellites loci was 0.62.
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