Aboshama,, H. (2006). MICROPROPAGATION AND SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY OF Justicr'a brandegeana. Journal of Plant Production, 31(8), 5267-5280. doi: 10.21608/jpp.2006.236127
H. M. S. Aboshama,. "MICROPROPAGATION AND SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY OF Justicr'a brandegeana". Journal of Plant Production, 31, 8, 2006, 5267-5280. doi: 10.21608/jpp.2006.236127
Aboshama,, H. (2006). 'MICROPROPAGATION AND SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY OF Justicr'a brandegeana', Journal of Plant Production, 31(8), pp. 5267-5280. doi: 10.21608/jpp.2006.236127
Aboshama,, H. MICROPROPAGATION AND SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY OF Justicr'a brandegeana. Journal of Plant Production, 2006; 31(8): 5267-5280. doi: 10.21608/jpp.2006.236127
MICROPROPAGATION AND SUITABLE PROTOCOL TO OVERCOME HYPERHYDRICITY OF Justicr'a brandegeana
Minufiya University. Genetic Engineering and Biotechnology Research Institute. Plant Biotechnology Department, Egypt.
Abstract
For micrOpropagation of Justicia brandegeana. shoot tips were excised from
mature plants grown in the greenhouse. These explants were cultured on MS medium
containing combination of BA and NAA. Hyperhydricity was a serious problem during
the micropropagation of Justicr‘a brandegeana. The effects of different factors for over—
coming of hyperhydricity were studied. This phenomenon was influenced by level and
type of cytokinin. salts media. vessel aeration and agar concentrations. High BA
concentrations (2.0 and 3.0 mgiL) gave multiple shoots but led to high incidence of
hyperhydricity. WPM was significantly effective in controlling hyperhydricity in shrimp < /p>
plant compared with MS medium. The treatment of 8.0giL agar with opening jars
(weekly) gave the lowest hyperhydricity percentage (8.39%) without affecting shoot
multiplication (11.20 shootiexplant).
The developed shoots were transferred to half— MS medium for rooting. Two
types of auxin were tested; IBA or NAA each at 0.0. 0.5 and 1.0 mgiL with or without
3.0 gll. AC. The treatment of 1.0 mgiL NM and 3.0gr'L AC produced the largest
number of roots (4.60). The plantlets were transferred from the rooting medium and
cultured in 1:1 peatmoss: sand mixture. The survival percentage was 73% after 5
weeks from transplanting.
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