Hassan,, N., Ragab, M. (2006). IN VITRO PRESERVATION AND MOLECULAR CHARACTERIZATION OF THREE STRAWBERRY VARIETIES. Journal of Plant Production, 31(5), 3013-3023. doi: 10.21608/jpp.2006.236004
Nevin A. Hassan,; M. A. Ragab. "IN VITRO PRESERVATION AND MOLECULAR CHARACTERIZATION OF THREE STRAWBERRY VARIETIES". Journal of Plant Production, 31, 5, 2006, 3013-3023. doi: 10.21608/jpp.2006.236004
Hassan,, N., Ragab, M. (2006). 'IN VITRO PRESERVATION AND MOLECULAR CHARACTERIZATION OF THREE STRAWBERRY VARIETIES', Journal of Plant Production, 31(5), pp. 3013-3023. doi: 10.21608/jpp.2006.236004
Hassan,, N., Ragab, M. IN VITRO PRESERVATION AND MOLECULAR CHARACTERIZATION OF THREE STRAWBERRY VARIETIES. Journal of Plant Production, 2006; 31(5): 3013-3023. doi: 10.21608/jpp.2006.236004
IN VITRO PRESERVATION AND MOLECULAR CHARACTERIZATION OF THREE STRAWBERRY VARIETIES
1National Gene Bank and Genetic Resources. A.R.C. Giza , Egypt
2Strawberry and Nontraditional Crops Center' Agriculture faculty. Ain- Shams University.
Abstract
Low temperature preservation experiment was carried out with 3 strawberry
varieties. The Merstim tips were collected in June and cultured on medium contain
Knob salts , BAP10 mgfL, 30-glL sucrose and 6-gfL agars with pH5.T at 25°C then at
4 and 12 weeks plant materials were transferred to media of knob salts without
hormones and different concentrations of sucrose 0’20630'40 and 60 gfL. Two
different temperatures were evaluated for preservation 25 C and 5 °C either kept in
dark or 16I8 h light! dark. Preservation was checked after 2,5,9 and 12 months. .
Results showed that the 3 varieties could be preserved for 12 months with survival
percentage of over 50%. The molecular markers of the three varieties were detected
using lSSR analysis. The three pn‘mers detected polymorphisms among the three
varieties and revealed 11, 12 and 8 unique markers characterizing the different
varieties” The dendogramk constructed with ISSR data to study the distance between
them.
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