Ibrahim,, M., Khalil, M., Mosa, A., Mostafa, M. (2006). MOLECULAR ASPECTS OF SOME Macrophomr'na phaseolina ISOLATES PATHOGENIC T0 SUNFLOWER IN EGYPT. Journal of Plant Production, 31(5), 2827-2837. doi: 10.21608/jpp.2006.235979
M. M. Ibrahim,; M. A. Khalil; A. A. Mosa; M. H. Mostafa. "MOLECULAR ASPECTS OF SOME Macrophomr'na phaseolina ISOLATES PATHOGENIC T0 SUNFLOWER IN EGYPT". Journal of Plant Production, 31, 5, 2006, 2827-2837. doi: 10.21608/jpp.2006.235979
Ibrahim,, M., Khalil, M., Mosa, A., Mostafa, M. (2006). 'MOLECULAR ASPECTS OF SOME Macrophomr'na phaseolina ISOLATES PATHOGENIC T0 SUNFLOWER IN EGYPT', Journal of Plant Production, 31(5), pp. 2827-2837. doi: 10.21608/jpp.2006.235979
Ibrahim,, M., Khalil, M., Mosa, A., Mostafa, M. MOLECULAR ASPECTS OF SOME Macrophomr'na phaseolina ISOLATES PATHOGENIC T0 SUNFLOWER IN EGYPT. Journal of Plant Production, 2006; 31(5): 2827-2837. doi: 10.21608/jpp.2006.235979
MOLECULAR ASPECTS OF SOME Macrophomr'na phaseolina ISOLATES PATHOGENIC T0 SUNFLOWER IN EGYPT
1Plant Pathol. lnstit, Agric. Res. Centre, Giza, Egypt.
2Department of Plant pathology. Faculty of Agriculture, Ain Shams University, Shoubre EI-Kheima, Cairo.
Abstract
Macrophomina phaseoiina is one of the most prevalent sunflower
(Heiianthus annuus L.) pathogens in Egypt. Very little is known about the genetic
diversity of this pathogen. Mycelial compatibility test among 18 pathogenic isolates
obtained from sunflower plants, showing charcoal rot from different government,
showed that isolates of Beni-Sweif could be 2000 considered one mycelial
compatibility group. but the remaining isolates gave incompatible reaction.
Electrophoresis analysis of 808 protein content of mycelium of all isolates showed
the presence of common bands with molecular weights of 16-11 25-26. 28-29. 35-
36. 38-39, 4849. 52-53. 80-81, 74-?5, “(9-80 and 84-85 K.dal among them. These
common bands could be used in quantitative determination of such pathogen in soil.
RAPD—DNA analysis using two randomized primers. A (Si-CCCGTCAGCA-S“ ) and e
(5‘ -CCCGTCAGCA-3‘ ). showed very interesting figure. Four common bands were
obtained by primer A, and other three common bands were found with primer 8.
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