EVALUATION OF VEGETATIVE GROWTH AND GENETIC IDENTIFICATION OF SOME Cassia SPECIES GROWN IN EGYPT

Document Type : Original Article

Authors

1 Department of Forestry and Woody Trees, Horticulture Research Institute, Agricultural Research Centre, Giza, Egypt.

2 Department of Agricultural Botany, Faculty of Agriculture, Cairo University, Giza, Egypt.

Abstract

The present investigation was carried out at the Wire-House of Agricultural Botany Department, Faculty of Agriculture, Cairo University, Giza, Egypt during the two growing seasons of 2004 and 2005 to evaluate the vegetative growth of some Cassia species grown in Egypt; i.e., C. glauca Lam., C. corymbosa Lam., C. occidentalis L. and C. sophera L. In addition, electrophoretic identification of the investigated Cassia species was under consideration. SDS-polyacrylamide gel electrophoresis separation of total soluble proteins in seeds of the investigated  Cassia species was done at Central Laboratory of Agricultural Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
The obtained results revealed that the four investigated species of the genus Cassia showed significant differences in their vegetative growth characters (plant height, stem diameter, number of developed leaves per plant, shoot fresh weight    per plant and shoot dry weight per plant) in both studied seasons due to the effect of species and Cassia sophera exceeded all other investigated species in this respect followed by Cassia occidentalis. Data on biochemical analysis indicated that, polyacrylamide gel electrophoresis separation of total soluble proteins can be used  as a genetic finger print for identification, differentiation and comparison among       the four different species of the genus Cassia under investigation.

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