Badawi,, M., Taha, S., AI-Hamada, R. (2006). EFFECT OF SALINITY AND MUTAGENS ON IN VITRO PERFORMANCE OF POTATOES. Journal of Plant Production, 31(1), 369-381. doi: 10.21608/jpp.2006.235706
M. A. Badawi,; S. S. Taha; R. I. AI-Hamada. "EFFECT OF SALINITY AND MUTAGENS ON IN VITRO PERFORMANCE OF POTATOES". Journal of Plant Production, 31, 1, 2006, 369-381. doi: 10.21608/jpp.2006.235706
Badawi,, M., Taha, S., AI-Hamada, R. (2006). 'EFFECT OF SALINITY AND MUTAGENS ON IN VITRO PERFORMANCE OF POTATOES', Journal of Plant Production, 31(1), pp. 369-381. doi: 10.21608/jpp.2006.235706
Badawi,, M., Taha, S., AI-Hamada, R. EFFECT OF SALINITY AND MUTAGENS ON IN VITRO PERFORMANCE OF POTATOES. Journal of Plant Production, 2006; 31(1): 369-381. doi: 10.21608/jpp.2006.235706
EFFECT OF SALINITY AND MUTAGENS ON IN VITRO PERFORMANCE OF POTATOES
Vegetable Crops Department, Faculty of Agric., Cairo University, Giza
Abstract
This experiment was carried out to study the effectiveness of chemical mutagen sodium azide, SA at different concentrations 0,0.5,0.75 and 1 mM. Gamma irradiation at doses of 0, 10, 20, 30 and 40 Gy was also used to test its effect on improving salinity tolerance of the Spunta potato cultivar by shoot tips culture, as well as to produce plants grown under different levels of sea salt 0, 1000,2000,4000 and 8000 ppm, aiming to induce and select in vitro salt tolerant plant lines. Subcultures were done to the plantlets four times under sea salt concentrations , the obtained results indicated that gradual increase of sea salt levels was negatively correlated with all growth parameters (sur/ivai percentage, plant length and number of leaves), survival percentage was 99.72% at the 4th subculture. Exposure to gamma irradiation at low doses (10 and 20 Gy) either alone or combined with sea salt application had stimulative effects on growth parameters, meanwhile higher doses (30 and 40 Gy) adversely affected all the tested parameters. The concentration of 0.75 mM SA improved survival percentage while SA had negative effect on other growth parameters. Concerning RAPD (Random Amplification of Polymorphic DNA) genetic difference between treatments and the control plants were detected. However, the presence of polymorphic bands with three 10-mer primers, enables possible early detection of mutation in vitro.
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