AboShama, H., Hamza, E., Amer, E., Mamdouh, M. (2021). Effect of Modified Tissue Culture Medium by Nanomaterial on Microtuber Formation of Potato Cultivar Hermes. Journal of Plant Production, 12(11), 1221-1230. doi: 10.21608/jpp.2021.209330
H. AboShama; Ebtsam M. Hamza; Enass S. Amer; M. A. Mamdouh. "Effect of Modified Tissue Culture Medium by Nanomaterial on Microtuber Formation of Potato Cultivar Hermes". Journal of Plant Production, 12, 11, 2021, 1221-1230. doi: 10.21608/jpp.2021.209330
AboShama, H., Hamza, E., Amer, E., Mamdouh, M. (2021). 'Effect of Modified Tissue Culture Medium by Nanomaterial on Microtuber Formation of Potato Cultivar Hermes', Journal of Plant Production, 12(11), pp. 1221-1230. doi: 10.21608/jpp.2021.209330
AboShama, H., Hamza, E., Amer, E., Mamdouh, M. Effect of Modified Tissue Culture Medium by Nanomaterial on Microtuber Formation of Potato Cultivar Hermes. Journal of Plant Production, 2021; 12(11): 1221-1230. doi: 10.21608/jpp.2021.209330
Effect of Modified Tissue Culture Medium by Nanomaterial on Microtuber Formation of Potato Cultivar Hermes
Plant Biotechnology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt
Abstract
Nanoparticles (NPs)are involved in multiple areas, i.e, electronics, energy, medicine, life sciences, and agriculture.Induction and accelerating of multiplication and microtuber formation of potato plants (Hermes cultivar) by some treatments i.e. nano-nutrients, medium composition as well as explant type and cultivation orientation were examined in the current study. Datarevealed that bothtreatments of Murashige and Skoog medium (MS) +1.0mg/l benzyl adrnine (BA)+200mg/l thiamine and MS+2.0mg/l BA + 100mg/l thiamine significantly maximized the shoot proliferationcapacity. The highest shoot length was obtained from MS-free BA medium in combination with all thiamine concentrations. The shoot numbers were augmented when calcium (Ca2+) were substituted with 660 or 1320 μg /l Ca2+ NPs compared with MS (2.77,2.77 and 2.53, respectively), also, shoot length and node numberswas not affected by areplacement which means Ca2+ion could be substituted with CaNPs in MS medium without decreasing in potatoshoot proliferation. The promising results of substitution magnesium (Mg2+) with Mg2+NPs were recorded, where 185 or 375 μg /l Mg2+NPs gave anequal or augmented number of shoots relative tothose obtained on MS medium of Hermes with no significant difference between them, node numbers and shoot length were superior as a result of replacement with Mg2+NPs. Shoot tip necroses did not appear with Mg2+NPs. Generally, Mg2+NPs (375 μg/l) can replace the Mg2+ions in MS medium and enhance growth parameters of potato cultivar Hermes.
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