El-Shihy, O., Ghallab, A., Gad, M., Abdel Salam, R. (2007). IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L. Journal of Plant Production, 32(6), 4451-4474. doi: 10.21608/jpp.2007.208776
O. M. El-Shihy; A. M. Ghallab; Mervat M. A. Gad; Ranya R. Abdel Salam. "IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L". Journal of Plant Production, 32, 6, 2007, 4451-4474. doi: 10.21608/jpp.2007.208776
El-Shihy, O., Ghallab, A., Gad, M., Abdel Salam, R. (2007). 'IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L', Journal of Plant Production, 32(6), pp. 4451-4474. doi: 10.21608/jpp.2007.208776
El-Shihy, O., Ghallab, A., Gad, M., Abdel Salam, R. IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L. Journal of Plant Production, 2007; 32(6): 4451-4474. doi: 10.21608/jpp.2007.208776
IN VITRO SHORT-TERM GERMPLASM PRESERVATION OF Myrtus communis L
2Timber Trees Dept., Horticulture Research Institute, A. R. C., Giza, Egypt
Abstract
Shoot tips were obtained from Myrtus communis tissue cultured shootlets and in vitro preserved either by cryopreservation protocols (through vitrification, encapsulation-dehydration) or by cold preservation (4°C), as well as control storage at culture room conditions, however, nodal stem was involved in dehydration protocol in order to be cryopreserved. Preservation was adopted for 1, 2 and 3 months durations; yet, control treatment was prolonged for one year. Several cryoprotection trials were adopted, involving DMSO and/or PEG and glycerol, prior to vitrification protocol. Cryopreserved explant recovery was tested, as well as shootlets regeneration following preservation processes underwent several investigations. Shoot tips precultured on 0.18 M sucrose recorded higher recovered survival, shootlets number, length and leaves number than on a higher concentration and than on mannitol, either for 2 or 4 days, however, vitrification treatment induced no shootlets proliferation. Regenerated shootlets growth characters (survival %, shootlets number and dry weights) induced after preservation under growth chamber conditions (control) for one year, recorded the highest observed results. Similarly, chemical composition (chlorophyll a, total chlorophylls, carotenoids, total indoles, total free amino acids, total sugars and indoles/phenols ratio) as well as essential oil content and components were augmented due to preservation under control conditions; however, fresh weight, chlorophyll b, as well as total soluble phenols were low. RAPD analysis proved that preserved shootlets maintained their genetic stability through germplasm preservation methods.
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