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Abderrassoul, H., El-Shennawy, O., El- Mokadem, H. (2007). GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS. Journal of Plant Production, 32(6), 4155-4164. doi: 10.21608/jpp.2007.208753
Hagar A. Abderrassoul; Ola A. El-Shennawy; Hoda El- Mokadem. "GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS". Journal of Plant Production, 32, 6, 2007, 4155-4164. doi: 10.21608/jpp.2007.208753
Abderrassoul, H., El-Shennawy, O., El- Mokadem, H. (2007). 'GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS', Journal of Plant Production, 32(6), pp. 4155-4164. doi: 10.21608/jpp.2007.208753
Abderrassoul, H., El-Shennawy, O., El- Mokadem, H. GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS. Journal of Plant Production, 2007; 32(6): 4155-4164. doi: 10.21608/jpp.2007.208753

GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS

Article 1, Volume 32, Issue 6, June 2007, Page 4155-4164  XML PDF (615.61 K)
Document Type: Original Article
DOI: 10.21608/jpp.2007.208753
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Authors
Hagar A. Abderrassoul1; Ola A. El-Shennawy2; Hoda El- Mokadem2
1Department of Genetics, Fac. Of Agric., Alex. Univ., Alexandria, Egypt.
2Department of Floriculture, Ornamental, Horticulture and Garden design, , Fac. Of Agric., Alex. Univ., Alexandria, Egypt
Abstract
Chrysanthemum morifoliumis a perennial flowering plants  of high economic value , that is Propagated   vegetatively by taking cuttings since it has a strong sporophytic self- incompatibility
        system. For differentiating between cultivars, traditional methods,morphological characters were established. PCR based molecular marker assays including RAPD,were employed to study genetic diversity and phylogenetic relationships among ten Chrysanthemum cultivars.
                RAPD analysis using 10 random primers generated 112 discrete markers ranging from, ( 280-2660 bp ) in size. Ninety-one of these were polymorphic ( 81.3% ), with an average of 11.2 markers per primer. The primers; OPA-12,OPD-08,OPB-08, and OPC-14 , could be specific markers for the cultivars; Bari, Bari, Delianne and Grand-white,respectively.
                Cluster analysis of RAPD-data using the software program;  SPSS version 10 , revealed that the cultivars belonging to the same type, were genetically related more than the others.
                These results suggest that RAPD marker is a useful method for Chrysanthemum genetic diversity analysis and phylogenetic relationships. 
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