GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS

Abderrassoul, Hagar A.; El-Shennawy, Ola A.; El- Mokadem, Hoda

doi:10.21608/jpp.2007.208753

GENETIC DIVERSITY AND RELATIONSHIPS IN Chrysanthemum morifolium AS REVEALED BY RAPD MOLECULAR MAKER, AND SOME MORPHOLOGICAL CHARACTERS

Document Type : Original Article

Authors

¹ Department of Genetics, Fac. Of Agric., Alex. Univ., Alexandria, Egypt.

² Department of Floriculture, Ornamental, Horticulture and Garden design, , Fac. Of Agric., Alex. Univ., Alexandria, Egypt

10.21608/jpp.2007.208753

Abstract

Chrysanthemum morifoliumis a perennial flowering plants of high economic value , that is Propagated vegetatively by taking cuttings since it has a strong sporophytic self- incompatibility
        system. For differentiating between cultivars, traditional methods,morphological characters were established. PCR based molecular marker assays including RAPD,were employed to study genetic diversity and phylogenetic relationships among ten Chrysanthemum cultivars.
                RAPD analysis using 10 random primers generated 112 discrete markers ranging from, ( 280-2660 bp ) in size. Ninety-one of these were polymorphic ( 81.3% ), with an average of 11.2 markers per primer. The primers; OPA-12,OPD-08,OPB-08, and OPC-14 , could be specific markers for the cultivars; Bari, Bari, Delianne and Grand-white,respectively.
                Cluster analysis of RAPD-data using the software program; SPSS version 10 , revealed that the cultivars belonging to the same type, were genetically related more than the others.
                These results suggest that RAPD marker is a useful method for Chrysanthemum genetic diversity analysis and phylogenetic relationships.