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Khattab, E., EL-Shazly, M., Abdelkader, H. (2007). NATURAL INFECTION PEANUT WITH Peanut mottle virus (PEMV) IN EGYPT.. Journal of Plant Production, 32(4), 2511-2525. doi: 10.21608/jpp.2007.207492
Eman A. Khattab; Manal A. EL-Shazly; Hayam S. Abdelkader. "NATURAL INFECTION PEANUT WITH Peanut mottle virus (PEMV) IN EGYPT.". Journal of Plant Production, 32, 4, 2007, 2511-2525. doi: 10.21608/jpp.2007.207492
Khattab, E., EL-Shazly, M., Abdelkader, H. (2007). 'NATURAL INFECTION PEANUT WITH Peanut mottle virus (PEMV) IN EGYPT.', Journal of Plant Production, 32(4), pp. 2511-2525. doi: 10.21608/jpp.2007.207492
Khattab, E., EL-Shazly, M., Abdelkader, H. NATURAL INFECTION PEANUT WITH Peanut mottle virus (PEMV) IN EGYPT.. Journal of Plant Production, 2007; 32(4): 2511-2525. doi: 10.21608/jpp.2007.207492

NATURAL INFECTION PEANUT WITH Peanut mottle virus (PEMV) IN EGYPT.

Article 10, Volume 32, Issue 4, April 2007, Page 2511-2525  XML PDF (689.25 K)
Document Type: Original Article
DOI: 10.21608/jpp.2007.207492
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Authors
Eman A. Khattab; Manal A. EL-Shazly; Hayam S. Abdelkader
Virus and Phytoplasma Res. Dept, Plant Path. Res. Instit., (ARC), Giza, 12619, Egypt.
Abstract
A virus causing mottling, yellowing, necrosis, malformation and stunting on Arachis hypogaea L. plants was identified as Peanut mottle virus (PeMV) on the basis of host range, symptomatology, serology and RT-PCR.  The purified virus formed a single zone in the density gradient columns. The absorption spectrum of the purified virus had Amax at 260 nm and Amin at 245 nm. The A 260/280 and Amax/min ratios were 2.6 and 1.3, respectively. The estimated yield of the purified virus was 0.7 mg / 100g of peanut tissue. The virus particles had about 720-750 nm long and 13 nm width. Antiserum produced against PeMV had a titer of 1/1024 using indirect ELISA. Reverse transcription-polymerase chain reaction (RT-PCR) based method was developed for testing peanut (Arachis hypogaea L.) plants for infection by Peanut mottle virus (PeMV).  The PCR product (340 bp) of PeMV/CP was successfully amplified by using one set of specific primers designed from conserved sequences within the capsid region of the virus.  A good correlation was obtained between virus detection by RT-PCR method and virus detection from the same plants by enzyme-linked immunosorbent assay (ELISA).
Keywords
PeMV; electron microscopy; ELISA; TBIA; DBIA; and RT-PCR
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