ALTERNATION USING BACTERIAL SUPERNATANTS IN DATE PALM MICROPROPAGATION

Farrag, Hala M. A.; Eweda, Wedad E.; Guirgis, A. A.; Ibrahim, A. M.; El-Banna, A. A.; Ibrahim, I. A.

doi:10.21608/jpp.2007.206734

ALTERNATION USING BACTERIAL SUPERNATANTS IN DATE PALM MICROPROPAGATION

Document Type : Original Article

Authors

¹ Central Laboratory For Date Palm Res. and Development,Agric. Res. Center (ARC).

² Depart. of Agric. Microbiology, Faculty of Agric., Ain Shams Univ.

³ Genetic Engineering and Biotechnology Res.Inst.(GEBRI), Menofyia Univ.

10.21608/jpp.2007.206734

Abstract

The present investigation was aimed to use phytohormones produced by some bacterial strains like Azospirillum brasilense, Azotobacter chroococcum, Bacillus megaterium and Klebsiella pneumoniae instead artificial hormones as a protocol in date palm micropropagation through somatic embryogenesis induced from shoot tip or leaf primordial explants, which were isolated from offshoot apex. In order to test the possibility to use supernatant of the strains, which are auxin over producers and\or cytokinin over producers in culture medium composition according to development stages for maximizing yield and to study the effect on each stage, i.e., callus initiation, embryo formation, shooting and rooting stages. In general, results showed that Klebsiella and Azotobacter have an auxin effect but Azospirillum and Bacillus were more related to cytokinin nature.