BOTANICAL STUDIES ON THE MICROPROPAGATION OF DATE PALM (Phoenix dactylifera, L.) USING TISSUE CULTURE TECHINIQUE: І- EFFECTS OF ANTIOXIDANT; AOS ON BROWNING CONTROL AND RELATED COMPOUNDS.

Document Type : Original Article

Authors

1 Agric Botany Dept., Faculty of Agric., Mansoura Univ., Egypt

2 Genetic of Engineering and Biotech. Research Inst., Menofia Univ

Abstract

Antioxidants; ascorbic and /or citric acids in Bartamoda and sakkoty   cv(s) increased F.Wt of the initial explant, its growth value and  total phenols whereas decreased browning degree of the tissues and the adjacent medium as well as the concentrations of H2O2 and proline. AOs increased the concentrations of AsA and GSH in the developed  explant of both date palm (Phoenix dactylifera L,) cv(s). AsA showed an additive effects to that of citric on increasing non-enzymatic and enzymatic antioxidants concentrations in both cv(s) .
Bartamuda cv showed, in general, higher F.Wt as well as concentrations of total phenols, H2O2 and proline , than Sakkoty cv. However,concentrations of AsA and GSH as well as CAT, GPOD and SOD in Baramuda cv was found to be less than that of Sakkoty cv over all treatments. This indicated that Bartamuda cv has high ability to used AsA and GSH specially the latter, in the synthesis of thio containing  molecules, particularly salfhydryl-containin amino acids.

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