Mohamed, M., Shehab, G., Mahmoud, A., Amer, A. (2008). INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).. Journal of Plant Production, 33(6), 4089-4103. doi: 10.21608/jpp.2008.166612
M. K. Mohamed; G. G. Shehab; Abeer A. Mahmoud; Alia A. Amer. "INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).". Journal of Plant Production, 33, 6, 2008, 4089-4103. doi: 10.21608/jpp.2008.166612
Mohamed, M., Shehab, G., Mahmoud, A., Amer, A. (2008). 'INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).', Journal of Plant Production, 33(6), pp. 4089-4103. doi: 10.21608/jpp.2008.166612
Mohamed, M., Shehab, G., Mahmoud, A., Amer, A. INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).. Journal of Plant Production, 2008; 33(6): 4089-4103. doi: 10.21608/jpp.2008.166612
INFLUENCE OF DIFFERENT PLANT GROWTH REGULATORS ON CALLUS INDUCTION AND FLAVONOID CONTENTS IN ALFALFA (Medicago sativa L.).
2Biochemistry Department, Faculty of Agriculture, Cairo University, Giza, Egypt.
3Plant Biotechnology Research Lab, Faculty of Agriculture, Cairo University, Giza, Egypt.
Abstract
Alfalfa contains many important substances including flavonoids which play many diverse roles in plant. Alfalfa callus was induced on media containing 6- benzyl aminopurin (BA), α -Naphthalene acetic acid (NAA), 2, 4 Dichloro phenoxy acetic acid (2, 4-D) and kinetin. The best callus formation was obtained from the medium of 2 mgl-1 2, 4- D + 1.5 mgl-1 Kinetin; leaf was the best organ of alfalfa in production of fresh weight. The 2nd subculture was higher in growth characters and flavonoids production as compared to the 1st subculture. Flavonoid concentration was higher in shoot tip of 1st subculture and leaves in the 2nd subculture.
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