Abd El-Hadi, A., El-Adl, A., Kash, K., El-Diasty, M. (2008). FINGER PRINTING FOR SOME MAIZE INBRED LINES THROUGH RAPD-PCR TECHNIQUE. Journal of Plant Production, 33(5), 3405-3411. doi: 10.21608/jpp.2008.166407
A. H. Abd El-Hadi; A. M. El-Adl; Kawther S. Kash; M. Z. M. El-Diasty. "FINGER PRINTING FOR SOME MAIZE INBRED LINES THROUGH RAPD-PCR TECHNIQUE". Journal of Plant Production, 33, 5, 2008, 3405-3411. doi: 10.21608/jpp.2008.166407
Abd El-Hadi, A., El-Adl, A., Kash, K., El-Diasty, M. (2008). 'FINGER PRINTING FOR SOME MAIZE INBRED LINES THROUGH RAPD-PCR TECHNIQUE', Journal of Plant Production, 33(5), pp. 3405-3411. doi: 10.21608/jpp.2008.166407
Abd El-Hadi, A., El-Adl, A., Kash, K., El-Diasty, M. FINGER PRINTING FOR SOME MAIZE INBRED LINES THROUGH RAPD-PCR TECHNIQUE. Journal of Plant Production, 2008; 33(5): 3405-3411. doi: 10.21608/jpp.2008.166407
FINGER PRINTING FOR SOME MAIZE INBRED LINES THROUGH RAPD-PCR TECHNIQUE
Genet. Dept., Fac. of Agric., Mansoura University, Egypt.
Abstract
In this investigation 11 inbred lines of maize were used. These inbred lines were crossed among them to obtain 30 F1 hybrids according to factorial mating design. DNA finger printing was made by using RAPD-PCR for all 11 maize inbred lines. The two primers, XD8 and XD9 were used in this technique. The results showed similarity between inbred lines. The results also revealed the presence of three common bands between all inbred lines at primer XD8, while five common bands were obtained at primer XD9. Cluster analysis for similarity degree between the 11 parental inbred lines was done and showed similarity degree between the within inbred lines. Phylsgenetic analysis showed high similarity coefficient between the inbred lines 6, 4 and 2, 3. However, there were high genetic distances obtained between inbred lines No: 4, 5, 6, 7 and 11.
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