Hegazy, A. (2008). MICROPROPAGATION OF EGYPTIAN DATE PALM C.V SELMY THROUGH FLORAL BUDS CULTURE.. Journal of Plant Production, 33(4), 2803-2815. doi: 10.21608/jpp.2008.164923
A. E. Hegazy. "MICROPROPAGATION OF EGYPTIAN DATE PALM C.V SELMY THROUGH FLORAL BUDS CULTURE.". Journal of Plant Production, 33, 4, 2008, 2803-2815. doi: 10.21608/jpp.2008.164923
Hegazy, A. (2008). 'MICROPROPAGATION OF EGYPTIAN DATE PALM C.V SELMY THROUGH FLORAL BUDS CULTURE.', Journal of Plant Production, 33(4), pp. 2803-2815. doi: 10.21608/jpp.2008.164923
Hegazy, A. MICROPROPAGATION OF EGYPTIAN DATE PALM C.V SELMY THROUGH FLORAL BUDS CULTURE.. Journal of Plant Production, 2008; 33(4): 2803-2815. doi: 10.21608/jpp.2008.164923
MICROPROPAGATION OF EGYPTIAN DATE PALM C.V SELMY THROUGH FLORAL BUDS CULTURE.
Genetic Engineering and Biotechnology Research Institute (GEBRI), Plant Biotech. Sec., Minufiya University, Sadat City, Egypt
Abstract
The totally closed date palm (Phoenix dactylifera L) female spathe was surface sterilized by sprayed with ethyl alcohol 70 % prior to aseptic conditions transfer, then flamed. To minimize fast oxidative browning, inflorescence stalks were shortened down to 3 cm then immersed in sterilized antioxidant solution containing citric and ascorbic acids 150 mg/L each for 2 hours, prior to culture.
Modified MS medium supplemented with NOA (5 mg/L) + NAA (5 mg/L) + 2iP (1 mg/L) + BA (1 mg/L) was superior in explant survival %, callus formation %, creamy callus colour % and subsequently embryogenic callus formation % after 4 months of incubation. The modified medium (3/4 salts strength) supplemented with IBA (0.3 mg/L) and 2iP (0.5 mg/L) was recorded higher percentage values of embryos formation and the highest significant percentage values of number of embryos as well as embryos fresh weight (g) after 6 weeks of incubation. Embryos cultured on the previous medium in addition to putrescine (100 mg/L) obtained significant values of multiplication rate and growth value as well as total soluble protein and PAL activity after 3 weeks of incubation.
Individual shootlets cultured on basal MS medium (3/4 salts strength) supplemented with putrescine (100 mg/L) and IBA (0.5 mg/L) achieved the highest significant values of root formation %, number of roots and root length (cm) as well as PAL activity after 2 months of incubation.
On the other hand, using of soil culture containing compost and perlite (1:1, v/v) significantly recorded the highest survival percentage, number of leaves/plantlet and leaf length (cm) for plantlets after three months of acclimatization.
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