SALT TOLERANCE (STO), A STRESS-RELATED PROTEIN IN ARABIDOPSIS HAS A MAJOR ROLE UNDER OTHER STRESS CONDITIONS

El – Shami, M. I.; El – Danasory, M. M.; El - Hashash, E. F.; Zaazaa, E. I.; Hager, M. A.; El Kafafi, S.; Elmalky, M.

doi:10.21608/jpp.2009.119145

SALT TOLERANCE (STO), A STRESS-RELATED PROTEIN IN ARABIDOPSIS HAS A MAJOR ROLE UNDER OTHER STRESS CONDITIONS

Document Type : Original Article

Authors

¹ Agronomy Department, Faculty of Agriculture, Al-Azhar University, Nasr city, Cairo, Egypt

² Biochemistry Department Faculty of Agriculture, Al-Azhar University, Nasr city, Cairo, Egypt

³ Botany Department Faculty of Agriculture, Al-Azhar University, Nasr city, Cairo, Egypt

⁴ Agricultural Research Centre, Rice Research and Training Centre (RRTC), Sakha, Kafr El-Sheikh, Egypt

10.21608/jpp.2009.119145

Abstract

Recently, salt tolerance protein (STO) of Arabidopsis and rice were identified and confirmed by STO in yeast cell. Here, we present data of one STO family in Arabidopsis which protein was cloned, and the expression levels of the genes were analysed by Reverse transcriptase (RT)-PCR, using gene-specific primers. We examined the expression ofAtSTO mRNA under different stress conditions. The whole plants in age two weeks were treated by (1mM H₂O₂), NaCl 0, 100, 200, 400 mM of NaCl concentration and 20% for PEG and were incubated for different time. For Brefeldin A (BFA) treatments, were added to the Arabidopsis cells culture in three days growth at concentration of 10 mg/ml, and the samples were collected at different times from added BFA. we present data of one STO family in Arabidopsis which has Zinc-finger protein, and our result indicted that H₂O₂, BFA were inhibitor of the activity of AtSTO mRNA and H₂O₂ decrease the expression level of AtSTO mRNA. Generally the expression of STO in presence of different concentrations of NaCl and other stresses factors showed that STO protein play a major role to drought tolerance.

Keywords