EFFECT OF GROWING MEDIA AND NITROGEN SOURCES ON GROWTH, ALKALOIDS CONTENT AND SOME CHEMICAL CONSTITUENTS OF CATHARANTHUS ROSEUS PLANT

Document Type : Original Article

Authors

1 Medicinal and Aromatic Research Department, Hort. Res. Inst. , A.R.C.

2 Dept. of Horticulture , Faculty of Agriculture, Zagazig University

3 Dept. of Horticulture, Faculty of Agriculture, El-Azhar Universality, Assuit

Abstract

This study was carried out during the two successive seasons of 2007 and 2008 at the Experimental Farm of El-Kassasin Horticultural Research Station, Ismailia Governorate, to detect the effect of difference media culture and nitrogen sources and ratios and their interaction on  growth and  alkaloid content of Catharanthus roseus. Periwinkle plants were grown in nine different media types with nutrient solution during drip irrigation. Nitrogen was applied as constant concentration of 200 ppm using three different ratios between ammonical nitrogen and nitrate nitrogen as 100% NH4 + 0% No3, 50% NH4 + 50% No3 or 0% NH4 + 100% No3. Whereas , different media types used were peatmoss , sand , clay , vermiculite, peatmoss: sand (1:1, v:v), peatmoss: clay (1:1, v:v), peatmoss: vermiculite (1:1, v:v), sand: clay (1:1, v:v) or sand: vermiculite (1:1, v:v). Generally , the combination  peatmoss, peatmoss + sand or peatmoss + vermiculite medium and 0% NH4+100% No3 treatments were more effective in plant height, number of branches /plant fresh and dry weight of leaves/ plant, fresh and dry weight of plant , alkaloids content in leaves and chemical constituents in leaves0(N, T, K and nitrate).